abstract statement of problem: the viscosity of orthodontics adhesive primers is crucial for stability and correct brackets ‘position during bond up. an excessive adhesive flow during brackets seating could change the brackets’ position. objectives: the main purpose of this study was to investigate the viscosity of four orthodontic adhesive primers in two different temperatures. materials and m...

Name of Sequence. Chicken prolactin (PRL) gene. Genus and Species. Gallus gallus (chicken). Origin of Clones. Genomic DNA from Xing Hua Chinese native chickens was used as a template. Primers were designed based on exon/intron junctions of the genomic turkey PRL gene sequence (Kurima et al., 1995). Polymerase chain reaction (PCR) amplifications of the PRL gene were performed by using the follow...

OBJECTIVE To evaluate the clinical performance of APC™II Victory Series™ (3M Unitek) brackets in direct orthodontic bonding with and without the use of primer. DESIGN A single-operator, two-centre prospective, non-inferiority randomized controlled clinical trial. SETTING The Orthodontic departments at the Leeds Dental Institute and St Luke's Hospital, Bradford, UK. ETHICAL APPROVAL Ethica...

Primer Design Assistant (PDA) is a web interface primer design service combined with thermodynamic theory to evaluate the fitness of primers. It runs in a Linux-Apache-MySQL-PHP structure on a PC equipped with dual CPU (Intel Pentium III 1.4 GHz) and 512 Mb of RAM. A succinct user interface of PDA is accomplished by built-in parameters setting. Advanced options on 5' GC content, 3' GC content, ...

We have developed a new primer design strategy for PCR amplification of distantly related gene sequences based on consensus-degenerate hybrid oligonucleotide primers (CODEHOPs). An interactive program has been written to design CODEHOP PCR primers from conserved blocks of amino acids within multiply-aligned protein sequences. Each CODEHOP consists of a pool of related primers containing all pos...

This service integrates primer designing code from the popular Primer3 1 software with a specificity check that uses a custom BLAST search. Primer-BLAST eliminates the need to design primers at another site and then perform a search with those primers using the main NCBI BLAST service to check specificity. Moreover, Primer-BLAST can design primers that amplify only a particular splice variant o...

Polymorphism. A Hinf I PCR-RFLP was identified in the porcine leptin receptor ( LEPR) gene. Source and Description of Primers. Human cDNA (GenBank accession no. U43168) sequence was used to design primers to amplify porcine genomic DNA. Primer Sequences. Forward primer: 5¢GCATCCCATATCTGAACCC3¢; reverse primer: 5¢-CCACTTAAACCATAGCGAATC3¢.

Species and Locus. Pig Myogenin (MYOG). Source and Description of Primers. Oligonucleotide primers designed from pig cDNA sequence (GenBank accession no. U14331) were used to amplify a 1,644-bp fragment of the porcine MYOG gene. Primer Sequences. Forward primer: 5¢-TCT ATG ACG GGG AAA ACT AC-3¢; reverse primer: 5¢-TGG AGC CAG AGT GGT GTA TC-3¢.

II. STUDY DESIGN AND METHODOLOGY ..........................................430 A. Content Analysis.......................................................................431 B. Primer on the Doctrine of Equivalents.....................................432 C. Database Construction ............................................................435 D. Measurement Criteria..................................