Oat (Avena sativa cv Fulghum) fructan hydrolase was purified by ammonium sulfate precipitation and anion-exchange, hydrophobic interaction, and size-exclusion chromatography. The enzyme was purified to homogeneity as determined by the presence of a single band (43 kD) on a silver-stained sodium dodecyl sulfate-polyacrylamide gel. A mixture of /3-2,6-linked fructan (neokestin) isolated from oat ...

The understanding and control of early hydration of tricalcium silicate (C₃S) is of great importance to cement science and concrete technology. However, traditional characterization methods are incapable of providing morphological and spectroscopic information about in situ hydration at the nanoscale. Using soft X-ray spectromicroscopy, we report the changes in morphology and molecular structur...

Two procyanidin fractions, namely oligomers and polymers isolated from grape seed methanolic extract were characterized. Phenolic composition and procyanidin purity of these fractions were determined by normal-phase and reverse-phase HPLC, thioacidolysis-HPLC, ESI-MS analyses, formaldehyde-HCl precipitation and elemental analysis. Antioxidant activities of these fractions and other well-known a...

A novel high throughput method for synthesis and screening of customized protein-resistant surfaces was developed. This method is an inexpensive, fast, reproducible and scalable approach to synthesize and screen protein-resistant surfaces appropriate for a specific feed. The method is illustrated here by combining a high throughput platform (HTP) approach together with our patented photo-induce...

Polymerization of benzylthiocyanate on silver nanoparticles produces organic shell covered nanoparticles with controllable thickness in a one-pot process. Gram scale quantities of core–shell materials have been synthesized by this method. The methodology involves the addition of the precursor into Ag–citrate solution and the shell nucleates over a period of three weeks with complete precipitati...

We present a systematic study of self-cross-linked microgels formed by precipitation polymerization of oligo ethylene glycol methacrylates. The cross-linking density of these microgels and, thus, the network flexibility can be easily tuned through the modulation of the reaction temperature during polymerization. Microgels prepared in absence of any difunctional monomer, i.e. cross-linker, show ...

The synthesis of polymers with tailored reactive functionalities plays an important role in the development of various materials for industrial and biomedical applications. In addition, there is a need to develop chemistry that is based on the use of biodegradable and renewable resources.1 Appreciable amounts of natural polyesters occur in higher plants as structural components, for example, cu...

This paper describes general trends in the redox potential and solubility of a dimercaptan, 2,5,-dimercapto-1,3,4thiadiazole (DMcT), and its various derivatives in acetonitrile, and illustrates how these trends manifest in the cyclic voltammetry observed at glassy carbon electrodes. Attention is focused on the polymerization/depolymerization processes which are the origins of the excellent char...