14 day-old mouse ovarian tissue and preantral follicles isolated from same-aged mice were incubated in a simulated microgravity culture system. We quantitatively assessed follicle survival, measured follicle and oocyte diameters, and examined ultrastructure of the oocytes produced from the system. We observed decreased follicle survival, downregulation of expressions of proliferating cell nucle...

AMH is synthesized specifically by granulose cells of developing ovarian preantral and small antral follicles. Circulating AMH levels in women with PCOS are 2to 3-fold more compared to ovulatory women with normal ovaries [6,7], which denotes to the 2to 3-fold rise in the count of small follicles detected in PCOS. The excessive AMH has been hypothesized to decrease follicle sensitivity to FSH in...

Objective In the present study, the effects of alginate (ALG) concentration and ovarian cells (OCs) on the development and function of follicles were simultaneously evaluated. MaterialsAndMethods In the first step, preantral follicles (100-130 µm in diameter) were mechanically isolated from the ovaries of 2-week-old NMRI mice, encapsulated in the absence or presence of OCs in 0.5, 0.75 and 1% A...

Initiation of folliculogenesis through the induction of primordial follicle development in the ovary has an important role in determining the fertility and reproductive fitness of most mammalian species. The factors that control this critical process are largely unknown. The hypothesis tested in the current study was that kit-ligand/stem cell factor (KL) promotes the initiation and progression ...

Background: With all the recent advances in cancer treatments, many young cancer patients find themselves facing the prospect of losing their fertility after aggressive chemotherapy or radiotherapy. Cryopreservation of ovarian cortical tissue has emerged as a potential option to restore fertility in these young women. Materials and Methods: Because autotransplantation of cryopreserved ovarian c...