INTRODUCTION Hemolysis can occur during sample collection, handling and transport. It is more frequent when the non-laboratory staff performs sampling. The aim of this study was to assess nurses' knowledge on the causes of hemolysis and consequential impact on the laboratory tests results. Additionally, the differences in knowledge, related to work experience, professional degree and previous e...

A quantitative urea-based amyloid b (Ab)-sodium dodecyl sulfate-polyacrylamide gel electrophoresis with Western immunoblot (Ab-SDS-PAGE/immunoblot) reveals highly conserved and disease-specific Ab peptide patterns (Ab 1-37, 1-38, 1-39, 1-40, 1-42) in Alzheimer’sdisease (AD)patientsandnondementedcontrols. For further standardization of this method, we analyzed cerebrospinal fluid (CSF) of eight ...

To the Editor: Serum has proven a difficult matrix for mass spectrometry (MS)-based clinical proteomic profiling. Problems occur mainly as a result of preanalytical variables in sample handling and processing, causing substantial changes in MS peptide profiles (1 ) that can completely abolish meaningful data interpretation (2 ). The necessary rigorous standardization of sample collection and pr...

Introduction The total testing process, which begins with the order of the physician, includes the preanalytical, analytical and postanalytical phases and ends with the results ready for interpretation. To obtain a reliable test result requires detection of all steps however, to control the preanalytical stage is such a complex way that much of the steps are human dependent and out of the labor...

The use of plasma lactate to assess metabolic or circulatory impairment requires definition of critical preanalytical and analytical parameters. Stability has been documented for only 15 min after acquisition when samples were collected with fluoride and transported on ice. We examined time elapsed before analysis, storage temperature, and the antiglycolytic agent used to define preanalytical c...