In this study, we evaluated the potential effect of the method of recovery (artificial vagina or electroejaculation) on the production and quality of Guirra ram spermatozoa cryopreserved for the possible constitution of a sperm bank. In order to address this question, we evaluated the effect of semen collection method on fresh semen quality parameters, including: volume, concentration, producti...

Background: Frozen-thawed rooster semen is not reliable for use in artificial insemination in commercial stocks. Low density lipoprotein (LDL) has been assessed for effectiveness as a cryoprotectant in the extender to improve the quality of frozen-thawed rooster semen. Although LDL has been evaluated in a few studies in other species for semen cryopreservation, to date, no study has been conduc...

Two hours after semen collection, aliquots containing 6xlO 9 spermatozoa were centrifuged for 10 rain at 300 g. The seminal plasma was removed and the spermatozoa were resuspended to 5 ml with Beltsville F5 (BF5) extender. The semen was cooled gradually to 5 C over a 2-hr period, 5 ml of BF5 containing 2% glycerol was added and the semen was mixed and frozen immediately into pellets of 0.15 to ...

Despite the long established and widely practised technique of artificial insemination in cattle, specific interactions between mammalian spermatozoa and the female genital tract remain incompletely understood. The following essay summarises one current view of the progress of bull spermatozoa in the female tract relative to the time of ovulation. Drawing on this physiological background, modif...

The aim of this study was to compare computer assisted sperm analysis (CASA) results frozen thawed bull semen using three different chambers. Sixty samples were (37°C; 30 sec), extended in PBS (30×106 spermatozoa/mL; 37°C) and incubated 2 min). Each sample analyzed by CASA [total motility, progressive (pro)/ non-progressive/rapid/medium/slow movement spermatozoa, VCL, VSL, VAP, ALH, BCF, LIN, S...

An improvement in avian semen cryopreservation is essential and has the potential to improve the cryo-gene banking efficiency. This study compared two cryopreservation methods (slow freezing and vitrification) and the effect of different thawing/warming temperatures (5 ̊C, 25 ̊C and 41 ̊C) on Venda cockerel’s spermatozoa. Semen samples from Venda cockerels were diluted with modified Kobidil extend...

Male infertility and the poor quality of sperm seem to be influenced by oxidative stress. In particular, the reactive oxygen species (ROS) mainly produced by morphologically altered spermatozoa affect sperm motility, morphology, and integrity. The aim of this study was to evaluate the efficacy of Myo-Inositol (Myo-Ins) on a number of parameters such as viscosity and total and progressive motili...

Background: Since the deleterious effects of reactive oxygen species (ROS) during sperm cryopreservation can be overcome by antioxidants, post-thaw human sperm quality may perhaps profit from adding antioxidants to the extender media prior to cryopreservation. This study evaluated the effects of different concentrations of Trolox supplementation to cryo-protective media on post-thaw motility an...

Objective: The aim of this study was to ascertain the effects adding green tea extract (GTE) skim milk-egg yolk (SM-EY) extender on both quality post-thawed bull semen and pregnancy rates recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 5 years weighing 900 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, 0.15 mg GTE/100 mL then frozen. After weeks storag...

Sperm cryopreservation represents a useful tool in the management of reproduction in goat production. However, freezing and thawing produce physical and chemical stress on the sperm membrane that reduces their viability and fertilizing ability. In this study, firstly we evaluated the effects of reduced glutathione (GSH, 1 and 5mM) supplementation of the thawing extender on parameters of frozen-...