The objective of this retrospective study was to determine an optimal time point for vitrification of cleavage-stage human embryos. This study included patients who were undergoing day 2 or day 3 vitrified-warmed cleavage-stage embryo transfer at the In Vitro Fertilization (IVF) Programme of the Shanghai First Maternity and Infant Hospital, China, affiliated to the Tongji University School of M...

The experiment was conducted to investigate the Ovarian response and embryo quality using different doses of follicle stimulating hormone (FSH-P) in North Omani Cattle breed (NOC) in order to determine the suitable dose. Eighteen donor NOC were divided into three groups (G1, G2, and G3) comprising of six cows in each group and treated with 20 (G1), 25 (G2) and 30 mg FSH (G3). Ovarian response w...

Background: It is known that acetylation level of the nuclear histones in cloned embryos is lower compare to normally developed embryos. Histone deacetylas inhibitors (HDACi) with improvement of acetylation level in these embryos can affect embryo quality in pre-implantation stage and expression level of different genes especially developmental genes. Materials and Methods: In this research, SA...

Human chorionic gonadotrophin (hCG) represents the gold standard trigger of final oocyte maturation in controlled ovarian stimulation cycles. An alternative with gonadotrophin-releasing hormone agonist (GnRH-a) has been demonstrated to be effective GnRH antagonist (GnRH-ant) cycles, inducing a more physiologic LH and FSH surge, but enhancing lutheal insufficiency poorer pregnancy outcomes. Some...

Abstract Niedenzuella (Tetrapterys) multiglandulosa, a vine plant found in Brazil, has been correlated to outbreaks of poisoning cattle and buffaloes, generating economic losses related the death due heart failure, miscarriage, abortion, stillbirth, neonatal mortality. The aim this study was examine embryotoxic potential aqueous extract on vitro bovine embryos. In performed five replicates embr...

This study assessed the feasibility of using LeY glycan secretion level in human embryos as a method of judging embryo quality. Embryo culture media from patients receiving in vitro fertilization-embryo transfer was collected, and quality scores of embryos were recorded. Secretions of LeY in the culture media in different development stages (from 4-cell to 10-cell), embryos in the same developm...

Background: Infertility is the inability to achieve a clinical pregnancy after 12 months of regular and unprotected sexual activity. Increases in child-bearing delay maternal age at first significantly impact rise age-related infertility demand for treatment using assisted reproduction techniques (ART). As result, many women with low ovarian reserve poor response (POR) conventional stimulation ...

BACKGROUND Poor ovarian response and retrieval of no oocytes following ovarian stimulation for in vitro fertilization (IVF) is a challenging problem for both the patient and the clinician. FINDINGS Recent evidence indicates that folliculogenesis occurs in a wave-like fashion indicating that there are multiple follicular recruitment waves in the same menstrual cycle. This relatively new scient...

background: matrigel (extracellular matrix) can improve the growth of many cell types in vitro. objective: the aim of the present study was to determine the effect of matrigel on the development of 2-4 cells human embryos in culture. material and methods: surplus 2-4 cells human embryos, resulting from icsi, were divided into two groups (control and test). quality of embryos in both groups was ...

BACKGROUND In cases of male infertility, routine analysis for sperm characteristics is a poor predictive factor for the segmentation rate and embryo development in assisted reproductive technologies. It is assumed that epigenetic factors could have an influence on the embryo's quality. The aim of this work was to determine the relationship between sperm DNA methylation level and fertilization a...