Lumpfish are used routinely as cleaner fish to delouse farmed Atlantic salmon ( Salmo salar ) and often translocated globally. In September 2021, an aquaculture production business in Dorset, England experienced considerable mortalities consignments of lumpfish Cyclopterus lumpus imported from Norway. Following suspicion infection with virus (CLuV) by a veterinarian, commercial testing provided...

Reverse transcriptase-PCR (RT-PCR) is a powerful tool for gene expression analysis at the RNA level, primarily due to its sensitivity, speed, ease of use, and versatility. In a typical RT-PCR procedure, a reverse transcriptase is used to generate a complementary DNA (cDNA) copy of RNA molecules, followed by polymerase chain reaction (PCR) to amplify the cDNA. Since the PCR process part is usual...

The aim of the study was to present the usefulness of one tube reverse transcriptase polymerase chain reaction assay (RT-PCR) in comparison to virus isolation test (VI) in the detection of equine arteritis virus. The group of 82 semen samples taken from different stallions was examined. Equine arteritis virus was isolated in RK-13 cell monolayers from the seminal plasma of 11 animals. The same ...

Outbreaks of food- and waterborne gastroenteritis are being increasingly reported throughout the world. The analysis of environmental samples by newer diagnostic techniques such as reverse transcription-PCR (RT-PCR) amplification of nucleic acid has begun to identify human enteric viruses (predominantly "Norwalk-like" viruses [NLVs]) as the cause of many of these outbreaks. To streamline NLV de...

Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood...

INTRODUCTION Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). METHODS The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. RESULTS Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectiv...

Fluorescence-based reverse transcription real-time quantitative polymerase chain reaction (RT-QPCR) is a highly sensitive method for the detection and quantitation of mRNA. To control and correct for sample variability, some common housekeeping genes such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-actin, and ubiquitin are often used as endogenous standards. Other internal calibra...

enteroviruses are the causative agents of a number of diseases in humans. group b coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. genomic data of enteroviruses has allowed developing new molecular approaches such as nucleic acid sequence based amplification (nasba) for detection of such viruses. in this study, coxsackievirus b3 (cvb3) was de...

Abstract A multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) assay was developed to enable the simultaneous detection and differentiation of four viruses that infect passion fruit, citrus-associated rhabdovirus (CiaRV), East Asian Passiflora virus (EAPV), latent (PLV), Telosma mosaic (TeMV). The optimized parameters included primer concentration, annealing temperature...

To describe the characteristics of neonates born to mothers with confirmed Covid-19 infection. A descriptive retrospective study using medical record data patients treated in neonatal intensive care unit (NICU) Dr. Soetomo Regional General Hospital from January 1 July 24, 2021. total 174 were positive COVID-19 reverse transcription-polymerase chain reaction (RT-PCR) swab test results. 11 RT-PCR...