نتایج جستجو برای: polymerase chain reactionn

تعداد نتایج: 352457  

Journal: :iranian journal of pathology 2008
ali sadeghi alireza sobhani zahra etaati ali jahanlu mahnaz shiroodi

background and objective: to estimate the risk of human papilloma virus (hpv) infection for cervical malignancies, we conducted a case-control study in southern iran (hormozgan province). materials and methods: for this purpose,52 paraffin embedded blocks with exact diagnosis of cervical carcinoma(50 carcinomas and 2 carcinomas in situ) from 2001 to 2006 and 52 praffin embedded blocks of cervic...

Journal: :iranian journal of pathology 2015
masoumeh salehpour naser tayyebi meibodi roghayeh teimourpour adel ghorani-azam samaneh sepahi

background &objective: breast cancer is the most common female malignancy. detection of dna of human papillomaviruses (hpvs) in breast carcinomas suggests that the virus may play a role in the pathogenesis of this disease. the aim of this study was to evaluate the frequency of hpvs genotypes 6, 11, 16, 18 and 31 in paraffin-embedded tissue samples of invasive breast carcinomas. methods: thr...

Journal: :acta medica iranica 0
reza shahsiah department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran. alireza abdollahi department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran. farid azmoudeh ardalan department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran. mohammad taghi haghi-ashtiani pediatrics center of excellence, children's medical center, tehran university of medical sciences, tehran, iran. issa jahanzad department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran. mohsen nassiri toosi department of gastroenterology, school of medicine, tehran university of medical sciences, tehran, iran.

fluorescent monitoring of dna amplification is the basis of real-time pcr. absolute quantification can be achieved using a standard curve method. the standard curve is constructed by amplifying known amounts of standards under identical conditions to that of the samples.the objective of the current study is to propose a mathematical model to assess the acceptability of pcr resulys.four commerci...

Journal: :avicenna journal of clinical microbiology and infection 0
sahar rastyani department of microbiology, faculty of medicine, hamadan university of medical sciences, hamadan, ir iran mohammad yousef alikhani department of microbiology, faculty of medicine, hamadan university of medical sciences, hamadan, ir iran; brucellosis research center, hamadan university of medical sciences, hamadan, ir iran iraj sedighi department of pediatric, faculty of medicine, hamadan university of medical sciences, hamadan, ir iran sima kazemi department of microbiology, faculty of medicine, hamadan university of medical sciences, hamadan, ir iran hamed farhadi kohan department of microbiology, faculty of medicine, hamadan university of medical sciences, hamadan, ir iran mohammad reza arabestani department of microbiology, faculty of medicine, hamadan university of medical sciences, hamadan, ir iran; brucellosis research center, hamadan university of medical sciences, hamadan, ir iran; department of microbiology, faculty of medicine, hamadan university of medical sciences, hamadan, ir iran. tel: +98-9188662009, fax: +98-8138380130

conclusions campylobacter is responsible for some important clinical problems such as enteritis and is also associated with meningitis and hemolytic-uremic syndrome. it is imperative to monitor the prevalence and antibiotic resistance of campylobacter spp. as well as other the zoonotic bacteria. results twelve (10%) campylobacter spp. from 120 stool samples were isolated including c. coli and c...

Journal: :veterinary research forum 0
mansour mayahi department of clinical sciences, faculty of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran darioush gharibi department of pathobiology, faculty of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran rahim ghadimipour phd candidate of bacteriology, department of pathobiology, faculty of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran forough talazadeh department of clinical sciences, faculty of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran

ornithobacterium rhinotracheale (ort) is a bacterium associated with respiratory disease, growth retardation, decreased egg production and mortality in chickens and turkeys. the objective of this study was isolation, identification and evaluation of antimicrobial susceptibility of ort bacterium in slaughtered broilers chicken flocks based on cultural and molecular tests in khuzestan province, s...

Journal: :the iranian journal of pharmaceutical research 0
maryam rabiei 1- food and drug laboratory research center, food and drug organization, ministry of health and medical education, tehran, iran. 2-department of molecular biology, food and drug control reference laboratory, ministry of health and medical education, tehran, iran. mehrangiz mehdizadeh department of molecular biology, food and drug control reference laboratory, ministry of health and medical education, tehran, iran. hossein vahidi department of biotechnology, faculty of pharmacy, shahid beheshti university of medical sciences and health services, tehran, iran. mahmoud alebouyeh 1- food and drug laboratory research center, food and drug organization, ministry of health and medical education, tehran, iran. 2- department of molecular biology, food and drug control reference laboratory, ministry of health and medical education, tehran, iran.

detection of genetically modified organisms (gmos) in food is an important issue for all the subjects involved in food control and customer’s right. due to the increasing number of gmos imported to iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. in this study, following the extraction of genomic ...

Journal: :jundishapur journal of microbiology 0
ali karimi medicinal plant research center, shahrekord university of medical sciences, shahrekord, ir iran loghman salimzadeh medicinal plant research center, shahrekord university of medical sciences, shahrekord, ir iran; medicinal plant research center, shahrekord university of medical sciences, shahrekord, ir iran. tel:+98-3813334691, fax:+98-3813334911 nader bagheri department of immunology, school of public health, tehran university of medical sciences, tehran, ir iran

methods and materials: hepatitis b virus dna was detected by conventional pcr in some of the serum samples obtained from hbsag-positive chronic hepatitis b (chb) patients who were referred to health centers of shahrekord for routine monitoring of the disease. subsequently, using real-time pcr, the dna samples were used for genotyping and analysis of resistance to lamivudine. results: the dna wa...

Journal: :jundishapur journal of microbiology 0
mehdi vejdani department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran; department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran. tel: +98-8314274618-21, fax: +98-8314276477 rezaei mansour department of statistic and epidemiology, kermanshah university of medical sciences, kermanshah, ir iran yezdan hamzavi department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran sina vejdani department of bioscience, university of calgary, alberta, canada naser nazeri department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran ali michaeli department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran

background cryptosporidium spp. is a widespread protozoan parasite involving humans and animals. objectives this study was carried out to evaluate the immunofluorescence assay (ifa) and pcr results for more accurate diagnosis of faecal specimens. patients and methods forty six faecal human specimens of cryptosporidium oocysts were examined by pcr and ifa in calgary, canada. in statistical analy...

Journal: :nephro-urology monthly 0
morteza izadi health research center, baqiyatallah university of medical sciences, tehran, ir iran nematollah jonaidi jafari health research center, baqiyatallah university of medical sciences, tehran, ir iran abbas mahmoodzadeh poornaki department of parasitology, school of medicine, zanjan university of medical sciences, zanjan, ir iran javid sadraei parasitology department of medical school, tarbiat modares university, tehran, ir iran babak rezavand department of parasitology, school of medicine, zanjan university of medical sciences, zanjan, ir iran; department of parasitology, school of medicine, zanjan university of medical sciences, zanjan, ir iran. tel: +98-2414240301; ext: 3, fax: +98-2414249553 hamid reza mirzaei department of immunology, school of medicine, tehran university of medical sciences, tehran, ir iran

background toxoplasma gondii is an opportunistic parasitic organism causing infection in many mammals, including immunosuppressed patients. toxoplasmosis as an opportunistic infection is highly prevalent among patients receiving a kidney transplant. objectives the purpose of this study was to identify and determine the prevalence of toxoplasma gondii in clinical samples collected from patients ...

ژورنال: :طب انتظامی 0
سعیده مرادی saeedeh moradi department of animal scienceدانشگاه زنجان، گروه علوم دامی، زنجان حمزه آذری hamezeh azari department of animal scienceدانشگاه زنجان، گروه علوم دامی، زنجان ایرج جعفری انارکولی iraj jafari anarkooli department of anatomy, zanjan university of medical sciencesدانشگاه علوم پزشکی زنجان گروه علوم تشریحی بابک قاسمی پناهی babak qasemi-panahi department of animal scienceدانشگاه تبریز دانشکده کشاورزی گروه علوم دامی سمیه الهامی somayeh elhami department of animal scienceدانشگاه زنجان، گروه علوم دامی، زنجان علی فروهرمهر ali forouharmehr department of animal science, ferdowsi university of mashhad. mashhad, iran.دانشگاه فردوسی مشهد ، دانشکده کشاورزی گروه علوم دامی

مقدمه: آفلاتوکسین ها ترکیباتی سرطان زا هستند که توسط قارچ های aspergillus flvaus و a. parasiticus تولید می شوند. در میان آفلاتوکسین های شناخته شده سرطان زایی آفلاتوکسین b1 بیشتر از سایر آفلاتوکسین ها می باشد. این سم می تواند با آلوده کردن مواد غذایی وارد بدن انسان و درنهایت بافت پستان شود. دو ژن مؤثر در سرکوب تومور در پستان، ژن های brca1 و brca2 می باشند که بیان این ژن ها می تواند توسط آفلاتوکس...

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