background bacterial resistance to antibiotics has become a major source of concern for public health. pseudomonas aeruginosa strains are important opportunistic pathogens. these bacteria have a high resistance to a wide range of existing antimicrobials and antibiotics. objectives the present study was performed to evaluate the frequency of kpc in p. aeruginosa isolated from clinical samples of...

objectives the objective of this study was to develop the primers for single and/or multiplex pcr amplification assays for simultaneous identification of class a, class b, and class d carbapenem hydrolyzing β-lactamases in enterobacteriaceae and then to evaluate their efficiency. conclusions we have reported here a reliable method, composed of single and multiplex pcr assays, for screening all ...

conclusions the determination of serotype and surface proteins of gbs strains distribution would ‎be ‎relevant ‎for the future possible formulation of a gbs vaccine. patients and methods in this cross-sectional study, a total of 100 gbs strains isolated from 237 pregnant and 413 non-pregnant women were investigated for molecular capsular serotypes and surface protein genes using the multiplex p...

conclusions: pcr method is a valuable, cost-effective and alternative tool for quick diagnosis of active tuberculosis in different clinical specimens. results: the concordance rate between the three sets of primers was calculated and is6110/buffer pcr method showed good agreement with the lj culture method (κ = 0.627, p < 0.0001). the sensitivity of is6110/buffer pcr was 58.33%, with specificit...

background the occult hepatitis b infection (obi) is defined as the presence of hepatitis b virus (hbv) dna in the sera or in the liver biopsy and the absence of hepatitis b surface antigen (hbsag) by serological test. objectives the current study aimed to evaluate the occult hbv infection by polymerase chain reaction (pcr) and determine hbv genotyping among the patients with abnormal alanine t...

conclusions according to this research and prevalence of hhv-6 in children, evaluation of csf (detecting the hhv-6 dna by pcr) is recommended in patients younger than 13 months with possible encephalitis. results from a total of 114 patients, hhv-6 was detected in 10 (8.8%), 90% of which were boys with mean age 7.7 months and median of 7.5 months. no significant differences were found in clinic...

conclusions simpleprobe® real-time pcr is a fast and accurate technique qualified to detect vkorc1 and cyp2c9 snps. these results encourage taking further steps towards using vkorc1 and cyp2c9 allelic screening to prevent clinical complications due to resistance or sensitivity to warfarin as well as anti-coagulant dose adjustment. results the allelic frequencies for vkorc1 c1173t (cc, ct, tt) w...

background: listeria monocytogenes is one of the most important food-borne bacteria causing septicemia, meningitis and encephalitis in humans. the objective of this study was to evaluate the occurrence and antibiotic resistance of the bacterium in retail minced beef in ahvaz, south-west of iran. methods: in this survey, 150 samples of minced beef were randomly obtained from retail butcheries in...

considering the importance of theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of t. equi among dromedaries in central iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. for this purpose, a total of 161 blood samples from camels were examined in terms of t. equ...