نتایج جستجو برای: polymerarase chain reaction

تعداد نتایج: 627571  

Journal: :the iranian journal of pharmaceutical research 0
maryam rabiei 1- food and drug laboratory research center, food and drug organization, ministry of health and medical education, tehran, iran. 2-department of molecular biology, food and drug control reference laboratory, ministry of health and medical education, tehran, iran. mehrangiz mehdizadeh department of molecular biology, food and drug control reference laboratory, ministry of health and medical education, tehran, iran. hossein vahidi department of biotechnology, faculty of pharmacy, shahid beheshti university of medical sciences and health services, tehran, iran. mahmoud alebouyeh 1- food and drug laboratory research center, food and drug organization, ministry of health and medical education, tehran, iran. 2- department of molecular biology, food and drug control reference laboratory, ministry of health and medical education, tehran, iran.

detection of genetically modified organisms (gmos) in food is an important issue for all the subjects involved in food control and customer’s right. due to the increasing number of gmos imported to iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. in this study, following the extraction of genomic ...

Journal: :jundishapur journal of microbiology 0
ali karimi medicinal plant research center, shahrekord university of medical sciences, shahrekord, ir iran loghman salimzadeh medicinal plant research center, shahrekord university of medical sciences, shahrekord, ir iran; medicinal plant research center, shahrekord university of medical sciences, shahrekord, ir iran. tel:+98-3813334691, fax:+98-3813334911 nader bagheri department of immunology, school of public health, tehran university of medical sciences, tehran, ir iran

methods and materials: hepatitis b virus dna was detected by conventional pcr in some of the serum samples obtained from hbsag-positive chronic hepatitis b (chb) patients who were referred to health centers of shahrekord for routine monitoring of the disease. subsequently, using real-time pcr, the dna samples were used for genotyping and analysis of resistance to lamivudine. results: the dna wa...

Journal: :jundishapur journal of microbiology 0
mehdi vejdani department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran; department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran. tel: +98-8314274618-21, fax: +98-8314276477 rezaei mansour department of statistic and epidemiology, kermanshah university of medical sciences, kermanshah, ir iran yezdan hamzavi department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran sina vejdani department of bioscience, university of calgary, alberta, canada naser nazeri department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran ali michaeli department of parasitology and mycology, kermanshah university of medical sciences, kermanshah, ir iran

background cryptosporidium spp. is a widespread protozoan parasite involving humans and animals. objectives this study was carried out to evaluate the immunofluorescence assay (ifa) and pcr results for more accurate diagnosis of faecal specimens. patients and methods forty six faecal human specimens of cryptosporidium oocysts were examined by pcr and ifa in calgary, canada. in statistical analy...

Journal: :nephro-urology monthly 0
morteza izadi health research center, baqiyatallah university of medical sciences, tehran, ir iran nematollah jonaidi jafari health research center, baqiyatallah university of medical sciences, tehran, ir iran abbas mahmoodzadeh poornaki department of parasitology, school of medicine, zanjan university of medical sciences, zanjan, ir iran javid sadraei parasitology department of medical school, tarbiat modares university, tehran, ir iran babak rezavand department of parasitology, school of medicine, zanjan university of medical sciences, zanjan, ir iran; department of parasitology, school of medicine, zanjan university of medical sciences, zanjan, ir iran. tel: +98-2414240301; ext: 3, fax: +98-2414249553 hamid reza mirzaei department of immunology, school of medicine, tehran university of medical sciences, tehran, ir iran

background toxoplasma gondii is an opportunistic parasitic organism causing infection in many mammals, including immunosuppressed patients. toxoplasmosis as an opportunistic infection is highly prevalent among patients receiving a kidney transplant. objectives the purpose of this study was to identify and determine the prevalence of toxoplasma gondii in clinical samples collected from patients ...

Journal: :iranian journal of virology 0
m salehi-vaziri department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran sh monavari department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran m khalili research and clinical center of infertility, yazd, iran m shamsi-shahrabadi department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran h keyvani department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran h mollaei department of virology and antimicrobial resistance research center, faculty of medicine, tehran university of medical sciences, tehran, iran

background and aims: sexually transmitted infections (stis) are of major concern to clinicians and researchers in the field of reproductive medicine. many sti pathogens cause incurable and often fatal diseases, and have been transmitted through insemination procedures. the role of herpes simplex virus in male infertility has been investigated using the sensitive methods. the aim of this study w...

Journal: :jundishapur journal of microbiology 0
mohammad aghazadeh infectious and tropical disease research center, tabriz university of medical sciences, tabriz, ir iran; department of knowledge and information science, tarbiat modares university, tehran, ir iran hossein samadi kafil drug applied research center, tabriz university of medical sciences, tabriz, iran reza ghotaslou department of knowledge and information science, tarbiat modares university, tehran, ir iran mohammad asgharzadeh drug applied research center, tabriz university of medical sciences, tabriz, iran maryam moghadami department of knowledge and information science, tarbiat modares university, tehran, ir iran mohammad taghi akhi drug applied research center, tabriz university of medical sciences, tabriz, iran

conclusions the high prevalence of oxa β-lactamase and high genetic diversity of p. aeruginosa indicated that the resistance of p. aeruginosa might be expanding in our studied hospitals. background pseudomonas aeruginosa is main bacterial pathogen accountable for nosocomial infections. furthermore, it could potentially become resistant to β-lactams, aminoglycosides and fluoroquinolones antibiot...

Journal: :acta medica iranica 0
samileh noorbakhsh department of pediatric infectious diseases, hazrate rasool hospital, school of medicine, iran university of medical sciences, tehran, iran. mohammad farhadi department of ent, school of medicine, iran university of medical sciences, tehran, iran. azardokht tabatabaei department of pediatric infectious diseases, hazrate rasool hospital, school of medicine, iran university of medical sciences, tehran, iran. vida zarabi department of radiology, research center of pediatric infectious, school of medicine, iran university of medical sciences, tehran, iran.

chlamydia pneumoniae is a common respiratory pathogen which is often found in our paediatric populations. many patients with community-acquired pneumonia caused by c. pneumoniae have symptoms suggestive of sinusitis.the role of c. pneumonia in rhinosinusiits children (meanage =4.3 ±2.5year). this case control study was done in the pediatric and ent clinics of hazrat rasul hospital in tehran (20...

ژورنال: :مجله دانشکده پزشکی اصفهان 0
رزیتا یوسفیان دانشجوی کارشناسی ارشد، گروه میکروبیولوژی، دانشکده ی علوم زیستی، دانشگاه آزاد اسلامی، واحد فلاورجان، اصفهان، ایران وجیهه کرباسی زاده استادیار، گروه میکروبیولوژی، دانشکده ی علوم زیستی، دانشگاه آزاد اسلامی واحد فلاورجان، اصفهان، ایران شراره مقیم دانشیار، گروه میکروب شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

مقدمه: مقاومت آنتی بیوتیکی در بین عوامل باکتریایی ایجاد کننده ی عفونت بیمارستانی رو به گسترش است. اسینتوباکتر بومانی (acinetobacter baumannii) یکی از این عوامل است که مقاومت فزاینده ی آن به آنتی بیوتیک های رایج، درمان عفونت های ناشی از این باکتری را با مشکل روبه رو ساخته است. هدف از این مطالعه، شناسایی acinetobacter baumannii جدا شده از نمونه های بالینی به روش pcr (polymerase chain reaction) و ...

Journal: :iranian journal of veterinary research 2015
k. nehra r. rana k. n. viswas t. r. arun v. p. singh

although mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. to address this limitation, the authors developed and optimized species-specific primer pairs that target m. eguigenitalium rpob (rna polymerase b subunit) gene sequences. the specificity of the pcr assay developed in this stu...

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