DNA from a region downstream of and overlapping the polyketide synthase (PKS) gene cluster for jadomycin B biosynthesis in Streptomyces venezuelae was cloned and sequenced. Analysis of the nucleotide sequence located one complete ORF (ORF6), an incomplete one representing the 3' region of ORF4 in the PKS cluster, and a second incomplete one (ORF7). The deduced amino acid sequences for ORFs 6 an...

Two Streptomyces strains, named N11-26 and DC10-5, were isolated from deep-sea non-photosynthetic stony coral, respectively. Strain produces lobophorin C divergolides, which are antimicrobial substances. This study aimed to classify these strains reveal their cryptic potential synthesize other secondary metabolites, such as polyketides nonribosomal peptides. Strains DC10-5 showed 16S rRNA gene ...

A rare actinomycetes strain of Saccharothrix texasensis, 6-C, has been isolated from the potato rhizosphere and it was shown to act as a biological control agent late blight. It is also first report on spp. inhibiting Phytophthora infestans. Here, we present complete genome data S. texasensis assembled by sequencing reads obtained both PacBio Illumina technologies with annotation. The final len...

Supernumerary mini-chromosomes–a unique type of genomic structural variation–have been implicated in the emergence virulence traits plant pathogenic fungi. However, mechanisms that facilitate and maintenance mini-chromosomes across fungi remain poorly understood. In blast fungus Magnaporthe oryzae (Syn. Pyricularia ), have first described early 1990s but, until very recently, overlooked studies...

The enoylreductase (ER) is the final common enzyme from modular polyketide synthases (PKSs) to be structurally characterized. The 3.0 Å-resolution structure of the didomain comprising the ketoreductase (KR) and ER from the second module of the spinosyn PKS reveals that ER shares an ∼600-Å(2) interface with KR distinct from that of the related mammalian fatty acid synthase (FAS). In contrast to ...