The flat gel acrylamide electrophoresis technique was standardized and applied to the comparison of four species of porcine mycoplasmas. Clear differences were observed between these species, and differences were seen among the strains of Mycoplasma hyosynoviae. The clarity of the patterns and the number of bands developed was influenced by the amount of protein in the extract and the age of th...

Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Common...

purification of drinking and industrial water required usage of high molecular weight polymer to cause flocculation process of dispersed suspension of contaminants. polyelectrolytes, including ionic polyacrylamide are especially appropriate for these purposes, because in this case the suspension stability can be controlled by both steric and electrostatic forces. thus the influence of solution ...

We describe a platform for high-throughput electrophoretic mobility shift assays (EMSAs) for identification and characterization of molecular binding reactions. A photopatterned free-standing polyacrylamide gel array comprised of 8 mm-scale polyacrylamide gel strips acts as a chassis for 96 concurrent EMSAs. The high-throughput EMSAs was employed to assess binding of the Vc2 cyclic-di-GMP ribos...

In current theories of polyacrylamide gel electrophoresis, the idea prevails that molecular sieving relies on different accessibility of volume fractions and of cross-sectional area fractions (denoted "pores") to different-sized ions due to the effect of "geometric exclusion". This correlates with the assumption that all elements of a polyacrylamide network occupy fixed and unchangeable positio...

Since its introduction by Robert Koch in 1882, agar has been the most commonly used substrate for the growth and study of bacteria. Agar consists of alternating blocks of D-galactose and 3,6-anhydro-L-galactose and is a polysaccharide with a variety of characteristics that are useful for culturing bacteria: (1) it is biocompatible; (2) it is inert to metabolism and degradation by bacteria; (3) ...

D ISCOT1XUOUS ELECT1IO1’HORESIS in polyacrylamide rods has been used for separation of serum proteins (1). In spite of satisfactory resolution in tile rod, the technic renhains cumbersome, and comparison of the closely migrating cOnlpoflents inaccurate. Akroyd (2) reported discontinuous electrophoresis On polyacrylamide gel slab, which alleviates many of the difficulties of the rod. We llave co...

background: detecting bence-jones protein in urine is essential for determining plasma cell dyscrasia and multiple myeloma. conventionally, acid-heat precipitation assay is used for detecting bence-jones protein in most medical laboratories; however, because of the low accuracy of this test, other more sensitive tests like urine electrophoresis are recommended.  materials and methods: in this s...

Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.

Twenty-three control samples of CSF were analyzed by vertical column electrophoresis on acrylamide gel. Quantitation results using a microdensitometer are presented. The normal protein pattern shows 15 constant bands which coincide in mobility and in many cases in staining pattern with serum proteins. The haptoglobin peaks, except for the band of the 1-1 type and the leading band of the 2-1 typ...