A double-isotope procedure was used to measure the effects of insulin and glucagon on ketone-body production and utilization (i.e. turnover) in the starved rat. Somatostatin was infused during the experiment to suppress the pancreatic release of either hormone. The immediate action of insulin in terms of ketone-body turnover that was most evident was a decreased production of 3-hydroxybutyrate,...

I. To determine the redox state of the mitochondrial NAD system in cirrhosis of the liver and in chronic quanti tat ive undelnutrition, the substrates of the Lglutamate dehydrogenase (EC 1.4.1.2 ) and D-fl-hydroxybutyrate dehydrogenase (EC 1.1.1.3o) reactions were measured. The livers of animals fed ad l ib i tum served as controls. 2. Assuming these substrates to be in equilibrium, the NAD+/NA...

The study addresses the growth of new wild-type strain Cupriavidus necator IBP/SFU-1 and synthesis poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB) on media containing fructose three different precursors 4HB (ε-caprolactone 1,4-butanediol 1,6-hexanediol). It was found that ε-caprolactone is best precursor for P(3HB-co-4HB) copolymers. By varying concentration number doses added into ...

Ralstonia eutropha H16 degraded (mobilized) previously accumulated poly(3-hydroxybutyrate) (PHB) in the absence of an exogenous carbon source and used the degradation products for growth and survival. Isolated native PHB granules of mobilized R. eutropha cells released 3-hydroxybutyrate (3HB) at a threefold higher rate than did control granules of nonmobilized bacteria. No 3HB was released by n...