نتایج جستجو برای: pld gene
تعداد نتایج: 1142850 فیلتر نتایج به سال:
Stimulation of phospholipase D (PLD) by cell surface receptors has been observed in many cell types. We have investigated the mechanism of activation of this enzyme in undifferentiated HL60 cells. GTP analogues and Ca2+ (buffered in the nanomolar to micromolar range) were introduced into HL60 cells in the presence of the permeabilizing agent, streptolysin O. We report that guanosine 5'-[gamma-t...
The ADP-ribosylation factor (ARF) GTP-binding proteins have been implicated in a wide range of vesicle transport and fusion steps along the secretory pathway. In chromaffin cells, ARF6 is specifically associated with the membrane of secretory chromaffin granules. Since ARF6 is an established regulator of phospholipase D (PLD), we have examined the intracellular distribution of ARF6 and PLD acti...
The effects of aminoglycoside antibiotics on phospholipase D (PLD) activity were investigated in permeabilized NG108-15 cells and in isolated rat brain membranes. Neomycin inhibited guanosine 5'-[gamma-thio]triphosphate-stimulated PLD activity in digitonin-permeabilized NG108-15 cells in a concentration-dependent manner (50% inhibition at 100 microM). Neomycin similarly inhibited PLD activity p...
Pulsed laser deposition (PLD) is a powerful technique for prototyping thin film materials, both single component (single composition) films and with varying composition (e.g., lateral continuous compositional spread, CCS). In this work, we improve one of the simulation methods used to design PLD films: We extend mathematical model material spread on substrate, T1(x,y), each pulse hitting target...
There is no optimal treatment for breast cancers lacking estrogen (ER) and progesterone (PgR) receptors in elderly women with co-morbidities that prevent use of "standard chemotherapy regimens" such as AC or CMF. The CASA trial studied pegylated liposomal doxorubicin (PLD) and low dose, metronomic cyclophosphamide + methotrexate (CM) for older (>65), vulnerable women with operable, ER and PgR-n...
During illumination, the light-sensitive plasma membrane (rhabdomere) of Drosophila photoreceptors undergoes turnover with consequent changes in size and composition. However, the mechanism by which illumination is coupled to rhabdomere turnover remains unclear. We find that photoreceptors contain a light-dependent phospholipase D (PLD) activity. During illumination, loss of PLD resulted in an ...
Phospholipase D (PLD), an enzyme widely distributed in bacteria, fungi, plants, and animals, catalyzes the hydrolysis of phosphatidylcholine (PC) and other phospholipids to generate phosphatidic acid (PA). PLD activity can lead to the generation of phosphatidylalcohol in the presence of a primary alcohol. This reaction, referred to as transphosphatidylation, is not only a hallmark of PLD activi...
In the accompanying paper (Chung, J.-K., Sekiya, F., Kang, H.-S., Lee, C., Han, J.-S., Kim, S. R., Bae, Y. S., Morris, A. J., and Rhee, S. G. (1997) J. Biol. Chem. 272, 15980-15985), synaptojanin is identified as a protein that inhibits phospholipase D (PLD) activity stimulated by ADP-ribosylation factor and phosphatidylinositol 4, 5-bisphosphate (PI(4,5)P2). Here, the purification from rat bra...
How to identify the drivers of population connectivity remains a fundamental question in ecology and evolution. Answering this can be challenging aquatic environments where dynamic lake ocean currents coupled with high levels dispersal gene flow decrease utility modern genetic tools. To address challenge, we used RAD-Seq genotype 959 yellow perch (Perca flavescens), species an ~40-day pelagic l...
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