نتایج جستجو برای: plasmid by the restriction enzymes then
تعداد نتایج: 17032277 فیلتر نتایج به سال:
the present study was conducted to investigate the effect of implicit focus on form through input flooding and the effect of noticing, explicit focus on form on linguistic accuracy. to fulfill the purpose of the study, 86 iranian pre-intermediate efl learners of one of the language institutes were chosen by means of administering ket as the homogeneity test. these learners were pretested throug...
جهت بهینه سازی شرایط تولید پروتئین هیدرولیز شده (شامل نسبت آنزیم به سوبسترا، دما و زمان) به منظور دستیابی به بالاترین خواص آنتی اکسیدانی، مینس تهیه شده از ضایعات میگوی ببری سبز (penaeus semisulcatus) پس از تعیین درصد پروتئین آن (13%، بر اساس وزن تر) با استفاده از دو پروتئاز تجاری آلکالاز و فلاورزایم بر اساس 18 تیمار تعیین شده با طرح آزمایشات سطح پاسخ برای هر آنزیم، هیدرولیز شده و با خشک کن انجم...
A protocol for increasing transcription from plasmid expression vectors is presented. A vector containing chloramphenicol acetyltransferase (CAT) gene was digested leaving the transcription cassette intact. Heat inactivation of restriction enzymes followed by ligation of the digestion products yielded concatemers which migrated as a single band in agarose gel electrophoresis. Mouse fibroblasts ...
peace and national security protection and ironic solving of international conflicts become the preliminary foundation of the united nations charter and had been considered as the main responsibilities of the security council, after world war ii and the establishment of united nations organization. therefore, the security council enables to analyze every kind of conflicts and challenges which ...
Background and Aim: Leishmania major p4 gene is normally expressed during amastigote form of the parasite and can be a good candidate for producing an effective vaccine. The aim of this study is to clone p4 gene in a suitable vector for further vaccine production studies .Materials and Methods: This is an experimental study. Leishmania promastigote was grown in N.N.N. medium and later in th...
1.1 introduction “i see translation as the attempt to produce a text so transparent that it does not seem to be translated. a good translation is like a pane of glass. you only notice that it’s there when there are little imperfections- scratches, bubbles. ideally, there shouldn’t be any. it should never call attention to itself.” “norman shapiro” (venuti, 1995:1) edward fitzgerald is the br...
The efficiency of conjugal transfer of plasmids from Escherichia coli to the cyanobacterium Anabaena sp. strain PCC 7120 was quantitated as a function of the number of restriction sites for the restriction enzymes carried by the recipient. In addition to the previously recognized isoschizomers of AvaI and AvaII, PCC 7120 was found to possess an isoschizomer of AvaIII. Plasmids modified in E. co...
this research study aimed to investigate the relationship between field-dependence/independence cognitive style and vocabulary learning strategies among iranian efl learners. ninety participants majoring in english translation at arak university were chosen. the participants were classified into two groups of field-dependent and independent based on the results of group embedded figure test (ge...
background: replacment of cd133+ cell’s β globin gene by using a gene targeting construct containing the β globin gene and essential elements for homologous recombination. materials and methods: pfbggt was amplified, then digested using the nhei and xhoi restriction enzymes, and finally, a 13.3 kb band (naked dna) was extracted from the agarose gel. biological activity of positive and negative ...
toxoplasmosis is an infection caused by the protozoan parasite toxoplasma gondii (t.gondii) throughout the world. although usually asymptomatic, the infection can cause serious medical problems in immunocompromised individuals and fetuses. toxoplasmosis also causes considerable economic loss because of abortion in livestock. dna vaccination is a promising approach against intracellular parasite...
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