Establishing an aseptic in vitro culture for Dendrobium, or for any plant in fact, is the most important step towards developing an effective in vitro tissue culture including micropropagation protocol. Success in initial aseptic culture will contribute to the successful production of in vitro cultures that may involve the initiation or formation of callus and/or protocorm-like bodies (PLBs), t...

Solanum lycopersicum cv. (Punjab Kesar Cherry) (Tomato) belonging to family Solanaceae has been priced for the presence of lycopene, low sugar content and curative properties. However, productivity tomatoes observed be due both biotic as well abiotic stress. Considering toxicity copper (Cu), lead (Pb) nickel (Ni) environmental contamination economic importance S. lycopersicum, present study was...

An experiment was carried out in Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to investigate the effect of banana extract on micropropagation of Dendrobium sp. var. Sonia orchid through PLBs. The experiment was conducted during July 2012 to October 2013. Half-Murashige and Skoog (1/2MS) medium were used as basal medium and the medium...

Valeriana officinalis is an important medicinal herb commonly found in Kashmir valley. This study forms an important preliminary step for in-vitro micro propagation of V. officinalis from breaking the seed dormancy, inducing rapid seed germination and its subsequent micro propagation. We investigated the influence of pretreatment of V. officinalis seeds with reduced temperature and light on see...

Cut flower ornamental kale (Brassica oleracea var. acephala) is a biennial cultivar, which completes sexual reproductive generation in two years. Isolated microspore culture (IMC) can accelerate plant homozygosity instead of self-pollinations. However, the application IMC cut was rare since its low rate embryogenesis. It proved that histone acetylation might affect gene expression microspores a...

A procedure was developed for in vitro propagation of Curculigo latifolia through shoot tip culture. Direct regeneration and indirect scalp induction of Curculigo latifolia were obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explant...

This research aimed to induce in vitro and identify polyploidy plantlets of Porang (Amorphophallus muelleri Blume) after colchicine treatment. In shoot base explants porang were cultured on MS medium containing different concentrations 30, 60, 90, 120 mg/l for four weeks while the control was without colchicine. After weeks, all treated untreated multiplied regenerated into plantlets. Flow cyto...