نتایج جستجو برای: pichia sp

تعداد نتایج: 124006  

Journal: :FEBS letters 1998
U Hochstrasser M Lüscher C De Virgilio T Boller A Wiemken

The cDNA encoding sucrose-fructan 6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) has been expressed in the methylotrophic yeast Pichia pastoris, using a translational fusion into vector pPICZ alphaC, containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor to allow entry into the secretory pathway. Transformed Pichia produced and secreted a functional 6-S...

Journal: :novelty in biomedicine 0
mehdi shokri immunology department and virology department, pasteur institute of iran, tehran, iran soheila ajdary immunology department, pasteur institute of iran, tehran, iran mina ebrahimi-rad biochemistry department, pasteur institute of iran, tehran, iran arash memarnejadian hepatitis and aids department, pasteur institute of iran, tehran, iran mohamad-hossein alimohamadian immunology department, pasteur institute of iran, tehran, iran fatemeh motevali hepatitis and aids department, pasteur institute of iran, tehran, iran

background: leishmania major lmsti1 is a conserved protein among different species of leishmania, and expressed in both amastigote and promastigote forms of l. major life cycle. it has previously been expressed in bacterial systems. m aterials and methods: to express lmsti1 in the methylotrophic yeast         pichia pastoris ( p. pastoris ), the shuttle vector ppicza containing gene lmsti1 was ...

2016
Blanca Iglesias-Figueroa Norberto Valdiviezo-Godina Tania Siqueiros-Cendón Sugey Sinagawa-García Sigifredo Arévalo-Gallegos Quintín Rascón-Cruz

In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf) in Pichia pastoris. After Bach fermentation and pu...

Journal: :Infection and immunity 2003
Anjali Yadava Christian F Ockenhouse

We have produced two synthetic genes that code for the F2 domain located within region II of the 175-kDa Plasmodium falciparum erythrocyte binding antigen (EBA-175) to determine the effects of codon alteration on protein expression in homologous and heterologous host systems. EBA-175 plays a key role in the process of merozoite invasion into erythrocytes through a specific receptor-ligand inter...

2015
Sara Epis Aida Capone Elena Martin Michela Paolucci Chiara Bazzocchi Matteo Valzano Jovana Bozic Stefano Novati Guido Favia Irene Ricci

2018
Yu-Chia Wei Stephanie Braun-Galleani Maria José Henríquez Sahan Bandara Darren Nesbeth

Transketolase is a proven biocatalytic tool for asymmetric carbon-carbon bond formation, both as a purified enzyme and within bacterial whole-cell biocatalysts. The performance of Pichia pastoris as a host for transketolase whole-cell biocatalysis was investigated using a transketolase-overexpressing strain to catalyze formation of l-erythrulose from β-hydroxypyruvic acid and glycolaldehyde sub...

Journal: :FEMS yeast research 2009
Heide-Marie Daniel Gino Vrancken Jemmy F Takrama Nicholas Camu Paul De Vos Luc De Vuyst

The fermentation of the Theobroma cacao beans, involving yeasts, lactic acid bacteria, and acetic acid bacteria, has a major influence on the quality of the resulting cocoa. An assessment of the microbial community of cocoa bean heap fermentations in Ghana resulted in 91 yeast isolates. These were grouped by PCR-fingerprinting with the primer M13. Representative isolates were identified using t...

Journal: :archives of razi institute 2016
d. shahbazzadeh m. azizy r. toroghi m.r. seyfi abad shapoori h. goudarzi

infectious bursal disease virus (ibdv) is the causative agent of gumboro disease, an infectious disease of global economic importance in poultry. the expression of heterologous proteins in p.pastoris is fast, simple and inexpensive. in this study, vp2 encoding gene of classical d78 ibdv was amplified using reverse transcription (rt) polymerase chain reaction (pcr) and cloned into ppiczαa vector...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید