نتایج جستجو برای: pichia pastoris gs115
تعداد نتایج: 4312 فیلتر نتایج به سال:
The first genome sequences of the important yeast protein production host Pichia pastoris have been released into the public domain this spring. In order to provide the scientific community easy and versatile access to the sequence, two web-sites have been installed as a resource for genomic sequence, gene and protein information for P. pastoris: A GBrowse based genome browser was set up at htt...
Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...
The bacteriocin enterocin A (EntA) produced by Enterococcus faecium T136 has been successfully cloned and produced by the yeasts Pichia pastoris X-33EA, Kluyveromyces lactis GG799EA, Hansenula polymorpha KL8-1EA, and Arxula adeninivorans G1212EA. Moreover, P. pastoris X-33EA and K. lactis GG799EA produced EntA in larger amounts and with higher antimicrobial and specific antimicrobial activities...
BACKGROUND Glycosides are compounds displaying crucial biological roles and plenty of applications. Traditionally, these molecules have been chemically obtained, but its efficient production is limited by the lack of regio- and stereo-selectivity of the chemical synthesis. As an interesting alternative, glycosidases are able to catalyze the formation of glycosides in a process considered green ...
BACKGROUND Talaromyces marneffei is an opportunistic dimorphic fungus prevalent in Southeast Asia. We previously demonstrated that Mp1p is an immunogenic surface and secretory mannoprotein of T. marneffei. Since Mp1p is a surface protein that can generate protective immunity, we hypothesized that Mp1p and/or its homologs are virulence factors. METHODOLOGY/PRINCIPAL FINDINGS We examined the pa...
Raw starch degrading enzyme specially glucoamylase with binding domain (SBD) has great values in the processing industry because it digests particles below gelatinization temperature by releasing glucose from non-reducing ends sequentially. The purpose of study was to measure secretion levels recombinant Pichia pastoris, using α-mating factor signal peptide (α-MF) and native Aspergillus flavus ...
bioconversion of cellulosic material into bioethanol needs cellulase complex enzymesthat contain endoglucanase, exoglucanase and beta glucosidase. one of the most important organisms that produce cellulases is the filamentous fungi, trichoderma reesei which able to secrete large amounts of different cellulases. these enzymes are probably the most widely used cellulases industrially, however, th...
Background: Equine follicle stimulating hormone (eFSH) is a pituitary heterodimeric glycoprotein consists of noncovalently linked of generic alpha subunit and a hormone specific beta subunit. The molecular weights of the subunits are similar and about 16 KD. In general, FSH plays a key role in controlling vertebrate gonadal functions. In female mammals, ovarian maturation and follicular growth ...
We report the construction of a Pichia pastoris integrating vector which contains the inducible CUP1 promoter from Saccharomyces cerevisiae. We show that the promoter is indeed inducible by copper when used in P. pastoris and that the level of induction is dependent on the amount of copper in the medium.
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