The present article implies an extracellular phytase production by Aspergillus niger under submerged and solid fermentation. Physical and Chemical conditions tested for optimal production of phytase using the single variable mode optimization technique. A considerable higher phytase production was obtained using rice bran as solid substrate (17.8 U/ml & 28.6 U/ml) at pH 5, on 4 day of incubatio...

This experiment was conducted to investigate the effect of phytase supplementation in diets reduced amino acids ( AA ), crude protein CP and phosphorus P ) on growth performance, carcass traits, tibia ash broiler chickens. A total 2,240 unsexed Ross 308 chickens were used 56 floor pens with 40 birds each fed one eight dietary treatments seven replicates until 35 d age. positive control PC diet ...

A phytase was isolated and partially purified from the pollen of Lilium longiflorum Thumb. Optimum activity was at pH 8.0. The phytase was activated by Ca(2+) and Sr(2+) but not by the other divalent cations tested. Activity was inhibited by ethylenediaminetetraacetate. The phytase had a temperature optimum of 55 to 60 degrees C and an activation energy of about 12,700 calories/mole. Extraction...

Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 2(2) full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% suc...

OBJECTIVE The objective of this study was to evaluate increasing doses of a novel microbial phytase (Cibenza Phytaverse, Novus International, St. Charles, MO, USA) on standardized total tract digestibility (STTD) of P in canola meal (CM), corn, corn-derived distiller's dried grains with solubles (DDGS), rice bran (RB), sorghum, soybean meal (SBM), sunflower meal (SFM), and wheat. METHODS Two ...

The aim of the study was to observe the effects of dietary available phosphorus (aP) and calcium (Ca), with regular or super doses of phytase, on phytate hydrolysis and subsequent influences on broiler growth performance and nutrient utilization. In a 2 × 3 factorial design, 384 Ross-308 broilers were allocated to one of 6 dietary treatments with 8 replicates in a randomized complete block desi...

The objective of this study was to investigate the effects of supplementation with free myo-inositol (MI) or graded levels of phytase on inositol phosphate (InsP) degradation, concentrations of MI in the digestive tract and blood, bone mineralization, and prececal digestibility of amino acids (AA). Ross 308 broiler hatchlings were allocated to 40 pens with 11 birds each and assigned to one of 5...

A trial was conducted to evaluate pure NSP enzyme combination derived from in vitro studies and commercially available phytase to corn-soybean meal based low energy diets singly and combination of both. The experiment was conducted by using completely randomized design on one hundred and fifty layer birds (40 weeks) of uniform body weight and production with five treatments, six replicates and ...

Supplementation with phytase is an effective way to increase the availability of phosphorus in seed-based animal feed. The biochemical characteristics of an ideal phytase for this application are still largely unknown. To extend the biochemical characterization of wild-type phytases, the catalytic properties of a series of fungal phytases, as well as Escherichia coli phytase, were determined. T...