Ena/VASP (vasodialator-stimulated protein) proteins regulate many actin-dependent events, including formation of protrusive structures, fibroblast migration, neurite extension, cell-cell adhesion, and Listeria pathogenesis. In vitro, Ena/VASP activities on actin are complex and varied. They promote actin assembly, protect filaments from cappers, bundle filaments, and inhibit filament branching....

In the preceding paper, we demonstrated that the endogenous phosphorylation of a protein with a molecular weight of 29,000 was enhanced by various secretagogues in rat pancreatic and parotid lobules, the phosphorylation of this protein correlating both temporally and in a dose-dependent fashion with secretory protein discharge. In the present study, we established a specific methodology to char...

Ca has been found to increase the quantity of (32)P incorporated into red cell ghosts from [gamma-(32)P]ATP over the levels obtained by incubation with Mg alone or with Mg + Na, in correlation with the effect of Ca on the associated ATPase activities. When the (32)P-labeled ghosts were solubilized in sodium dodecyl sulfate (SDS) and electrophoresed on acrylamide gels only two bands could be det...

Phosphorylation is an important post-translational protein modification with regulatory roles in diverse cellular signaling pathways. Despite recent advances in mass spectrometry, the detection of phosphoproteins involved in signaling is still challenging, as protein phosphorylation is typically transient and/or occurs at low levels. In green plant tissues, the presence of highly abundant prote...

Cell motility requires lamellipodial protrusion, a process driven by actin polymerization. Ena/VASP proteins accumulate in protruding lamellipodia and promote the rapid actin-driven motility of the pathogen Listeria. In contrast, Ena/VASP negatively regulate cell translocation. To resolve this paradox, we analyzed the function of Ena/VASP during lamellipodial protrusion. Ena/VASP-deficient lame...

Neurexins (NXs) and neuroligins (NLs) are cell adhesion molecules that are localized at opposite sites of synaptic membranes. They interact with each other to promote the assembly, maintenance, and function of synapses in the central nervous system. Both NX and NL are cleaved from a membrane-attached intracellular domain in an activity-dependent manner, generating the soluble ectodomain of NX o...

conclusions the results of this work demonstrated that the subcellular distribution of pfdn1 was altered in the rabv-infected n2a cells and colocalized with the n protein of rabv in the nbl structures. results confocal microscopy showed that pfdn1 was colocalized with the n protein of rabv in the infected n2a cells and was mainly recruited to the characteristic negri-body-like (nbl) structures ...

esophagus cancer is the eighth most common cancer worldwide and particularly high in an area extending from the southern border of the caspian sea in iran across central asia to china. since information about this mysterious disease is poor, proteomics may be solving this enigma. altering gene expression in cancer cell is a remarkable indicator can be detected by proteomics techniques and bioin...

The immunohistochemical detection and quantification of phosphoprotein levels can provide valuable information regarding the activation of cell signaling pathways within tumor samples, with potential implications for prognosis and treatment selection. However, phosphoprotein levels may be affected by clinical tissue handling, hypoxia and other cellular stressors prior to tissue fixation. The pr...

Intensive researches in seismology and earthquake engineering are ongoing in Korea since the mid 1990s.This paper summarizes recent R&D; results regarding the improvement of earthquake catalog, seismicityand tectonics, ground motion, and PSHA methodology. The effect of the R&D; results on the seismichazard was studied for the Ulchin NPP site. Results showed much improvement in the...