Reportedly, determination of several phospholipids in amniotic fluid, including phosphatidylglycerol and phosphatidylinositol, in addition to lecithin and sphingomyelin improves prediction of neonatal pulmonary function. In this study, we evaluated a two-dimensional technique for separating and measuring these phospholipids and compared it with a simpler one-dimensional procedure. The two-dimen...

Acetylation of Atg3 regulates the lipidation of the protein Atg8 in autophagy. The molecular mechanism behind this important biochemical event remains to be elucidated. We describe the first semi-synthesis of homogeneous K19/K48-diacetylated Atg3 through sequential hydrazide-based native chemical ligation. In vitro reconstitution experiments with the semi-synthetic proteins confirm that Atg3 ac...

‘THE LENGTHY INCUBATION PERIOD and the subjective interpretation of degree of flocculation ai’e undesirable characteristics of the Hanger cephalin cholesterol flocculatiomi test (1). Several workers (2-4) have reported modifications either to shorten test time or to determine extent of flocculation mnstrumemitally. Inadequate sensitivity has been noted in some of these technics as well as inter...

The synthesis of two new synthetic analogues of lecithin, two of phosphatidyl ethanolamine ("cephalin"), and one new phosphatidic acid analogue is described. They comprise one of each of the following types: the "isosteric" diether lecithin and cephalin analogues ROCH(2)CH(OR)- CH(2)CH(2)P(O) (O(-))OCH(2)CH(2)N(+)R'(3) (R = C(18)H(37); R' = H or CH(3)); and the "hydrocarbon" analogues of phosph...

Oriented films and powder samples of the phospholipid L-alpha-dipalmitoyl phosphatidylethanolamine (DPPE) were studied by X-ray fiber diffraction techniques. The specimens were photographed under a complete range of hydration at room temperature. Two polymorphic forms were found. One of them was present in oriented films. It showed a close packing of DPPE molecules lying parallel to the normal ...

OBJECTIVE To describe and analyze indicators of feeding practices related to breastfeeding and factors associated with exclusive breastfeeding (EBF) in a subnormal urban cluster (slums) in Pernambuco, Brazil. METHODS Four breastfeeding indicators were used to interview mothers of children under 3 years of age. An inventory of the families' socioeconomic and environmental factors, maternal obs...

Basic relationships between the phase diagrams, previously considered independent of each other, are described. Phase diagrams of two-component phosphatidylcholine/phosphatidylcholine (PC/PC), phosphatidylethanolamine/phosphatidylethanolamine (PE/PE), and PC/PE lipid membranes are systematically investigated by means of the Landau theory. While gradually changing the chain length of one of the ...

Listeners have a remarkable ability to localize and identify sound sources in reverberant environments. The term "precedence effect" (PE; also known as the "Haas effect," "law of the first wavefront," and "echo suppression") refers to a group of auditory phenomena that is thought to be related to this ability. Traditionally, three measures have been used to quantify the PE: (1) Fusion: at short...