* For DPN synthesis, 1.0 ml. of reaction mixture contained 50 y of the enzyme preparation, 0.3 micromole of MgCl 2, and 50 micromoles of glycylglycine buffer (pH 7.4) in addition to ATP and NMN; for ATP synthesis 1.0 ml. contained 50 y of the enzyme preparation, 0.75 micromole,of MgC12, and 50 micromoles of glycylglycine buffer (pH 7.4) in addition to DPN and P-P. Constant values were reached a...

Objective Anti-Rh D IgG is used for the prevention of anti-D antibody production in Rh- individuals who have been exposed to Rh+ red blood cells. The stability of IgG preparations as a solution is low, with a shelf life of a year or more. Formulation of anti-Rh D IgG as a lyophilized preparation would decrease its degradation rate and increases its shelf life. The objective of this study was t...

Conjugation of methotrexate (MTX) (MW 454) with different molecular weights of polyethylene glycol (PEG) including methoxy-peg (mpeg) 750 D and 5000 D and diol-peg 35000 D led to compounds that are physicochemically highly different from the parent compound, MTX. In this study, an HPLC system consisting of C8 column and UV detector (?=342 nm), using a mixture of 30:70 v/v phosphate-citrate buff...

A method utilizing bovine serum albumin (BSA) as buffer additive for chiral separation by means of capillary electrophoresis is described. Parameters that affect chiral separation, such as buffer pH, buffer concentration, BSA concentration, and organic modifier, are investigated. Baseline resolution of ephedrine-pseudoephedrine and norephedrine-norpseudoephedrine isomers are achieved in an unco...

A B S T R A C T Although pyridoxal phosphate is known to inhibit gelation of purified hemoglobin S, antisickling activity has never been demonstrated for intact erythrocytes. We incubated washed erythrocytes at 370C either in buffer alone, or with added pyridoxal phosphate or pyridoxal, washed these cells, suspended them in untreated buffer, and compared the percent modified hemoglobin, the oxy...

Catalase, one of the antioxidant enzymes, decomposes hydrogen peroxide into water and oxygen. From discovery catalase, several studies have been carried out to reveal its importance in health, food cosmetics industries etc., these are still ongoing. In this study, catalase enzyme was partially purified from muscle tissue Dusky spinefoot (Siganus luridus). Purification procedure consisted homoge...

A vancomycin (Van) derivative self-assembles in a phosphate buffer as a divalent Van and on cell surfaces as a multivalent Van, which offers potent activity against VRE.

ZnO activation of metallic Al for generating electricity for bioelectronic applications. Selective electrocatalysis in phosphate rich electrolyte. A cell operating in physiological saline buffer produces an open-circuit

conjugation of methotrexate (mtx) (mw 454) with different molecular weights of polyethylene glycol (peg) including methoxy-peg (mpeg) 750 d and 5000 d and diol-peg 35000 d led to compounds that are physicochemically highly different from the parent compound, mtx. in this study, an hplc system consisting of c8 column and uv detector (?=342 nm), using a mixture of 30:70 v/v phosphate-citrate buff...

Conjugation of methotrexate (MTX) (MW 454) with different molecular weights of polyethylene glycol (PEG) including methoxy-peg (mpeg) 750 D and 5000 D and diol-peg 35000 D led to compounds that are physicochemically highly different from the parent compound, MTX. In this study, an HPLC system consisting of C8 column and UV detector (?=342 nm), using a mixture of 30:70 v/v phosphate-citrate buff...