Abstract Bacteria persist under constant threat of predation by bacterial viruses (phages). Bacteria-phage conflicts result in evolutionary arms races often driven mobile genetic elements (MGEs). One such MGE, a phage satellite Vibrio cholerae called PLE, provides specific and robust defense against pervasive lytic phage, ICP1. The interplay between PLE ICP1 has revealed strategies for molecula...

Hepatitis C virus (HCV) infects ∼2% of the world's population. It is estimated that there are more than 500,000 new infections annually in Egypt, the country with the highest HCV prevalence. An effective vaccine would help control this expanding global health burden. HCV is highly variable, and an effective vaccine should target conserved T- and B-cell epitopes of the virus. Conserved B-cell ep...

Background and Objectives Cholera is a life-threatening diarrhea caused mainly by Gram-negative marine habitant Vibrio cholerae serogroup O1. Cholera vaccination is limited mainly to developed countries, due to the cumbersome and expensive task of vaccine production. In the present work, the aim was to study the immunogenicity of the synthetic mimotopes through two different routes of injection...

We report a de novo -assembled draft genome sequence of the Indian Staphylococcus aureus type 88 (ST88) strain LVP-7, isolated from an ocular infection. The harbors Panton-Valentine leukocidin phage, V staphylococcal cassette chromosome mec element, delta-hemolysin-converting Newman phage ΦNM3, and pathogenicity island SaPI3, encoding superantigen enterotoxin B.

Four groups of Dekalb Delta commercial layer hens (8 hens per group) were reared individually in cages in an isolation unit. At the age of 8 weeks, groups 1 and 2 were vaccinated with 9R Salmonella gallinarum live (9R live) vaccine. At the age of 18 weeks, group 1 was re-vaccinated with 9R live S. enteritidis vaccine, while groups 2 and 3 were vaccinated with S. enteritidis bacterin. At the age...

Tumor-targeted therapies are playing growing roles in cancer research. The exploitation of these powerful therapeutic modalities largely depends on the discovery of tumor-targeting ligands. Phage display has proven a promising high throughput screening tool for the identification of novel specific peptides with high binding affinity to cancer cells. In the present study, we describe the use of ...

Phage-derived affinity peptides have become widespread thanks to their easy selection via phage display. Interactions between a target protein and its specific peptide are similar those antibodies antigens. The strength of these non-covalent complexes may be described by the dissociation constant (Kd). In this paper, protein-specific exposed on pIII present in M13 bacteriophage virion with up f...

The rapid and specific point-of-care (POC) analysis of virulent pathogenic strains plays a key role in ensuring food quality safety. In this work, paper-based fluorescent phage biosensor was developed for the detection E. coli O157:H7 strain (as mode pathogens) samples. Firstly, phages that can specifically combine with (E. coli) were stained SYTO-13 dye to prepare novel probe (phage@SYTO). Sim...

Life-threatening infections caused by multidrug-resistant bacteria are becoming increasingly difficult to treat. There is growing interest in exploiting bacteriophages (or phages) combat bacterial infections. Phages often target surface structures that may also be important for virulence. Upon phage challenge, these molecules lost or modified, resulting resistance and possibly phenotypical conv...

Tumor-targeted therapies are playing growing roles in cancer research. The exploitation of these powerful therapeutic modalities largely depends on the discovery of tumor-targeting ligands. Phage display has proven a promising high throughput screening tool for the identification of novel specific peptides with high binding affinity to cancer cells. In the present study, we describe the use of ...