We have identified two Rhizobium meliloti genes (nodD2 and nodD3) that are highly homologous and closely linked to the regulatory gene nodD (nodD1). R. meliloti strains containing mutations in the three nodD genes in all possible combinations were constructed and their nodulation phenotypes were assayed on Medicago sativa (alfalfa) and Melilotus alba (sweet clover). A triple nodD1-nodD2-nodD3 m...

The present study investigated the impact of environmental pollution, represented in soil,irrigation water and air heavy metals, on the growth and production of Egyptian clovercultivated at south Greater Cairo, Egypt. Plants were sampled through five quadrats (0.5×0.5m), distributed equally in four cultivated farms in unpolluted and polluted sites, at theharvesting time. In addition, soil, air ...

The transcription of four members of the Kunitz proteinase inhibitor (KPI) gene family of white clover (Trifolium repens L.), designated as Tr-KPI1, Tr-KPI2, Tr-KPI4 and Tr-KPI5, was investigated at both local infection (roots) and systemic (leaf tissue) sites in white clover in response to infection with the clover root knot nematode (CRKN) Meloidogyne trifoliophila and the clover cyst nematod...

The data provide an overview of proteomic changes in red clover (Trifolium pratense L.) in response to cold acclimation and recurrent selection for superior freezing tolerance. Proteins were extracted from crowns of two red clover cultivars grown under non-acclimated or cold-acclimated conditions, and plants obtained from the initial genetic background (TF0) and from populations obtained after ...

Cell culture and genetic engineering techmques can be used to develop improved pasture plants. To utltise these methods we have developed procedures for regenerating plants from tissue cultures of perennial ryegrass and white clover. In both, the plant genotype influences regeneration capacity. There was significant genetic variation among regenerated perennial ryegrass plants in a wde range of...

The isolation of small amounts of genistein (5:7:4'trihydroxy-isoflavone) from subterranean clover (Trifolium subterraneum var. Dwalganup) and the demonstration of its weakly oestrogenic properties (Bradbury & White, 1951, and Biggers & Curnow, 1954), together with the difficulties in the synthesis of this compound, made it important that a more efficient method be found for the isolation of th...

Introduction: Breast cancer is one of the most common malignancies among Iranian women. Nowadays, the use of traditional medicinal plants has emerged as a tempting complementary to the treatment of breast cancer due to minimal side effects and less documented drug resistance. The aim of this study was to examine the anticancer effects of the essential oils of red clover and mature fruit of Bass...

Introduction: Breast cancer is one of the most common malignancies among Iranian women. Nowadays, the use of traditional medicinal plants has emerged as a tempting complementary to the treatment of breast cancer due to minimal side effects and less documented drug resistance. The aim of this study was to examine the anticancer effects of the essential oils of red clover and mature fruit of Bass...

Seeds of alfalfa (Medicago sativa) and white clover (Trifolium repens) were primed in 3 priming solutions [2% NaCl, 2% KNO3 and 300 g/L polyethylene glycol (PEG)] for 2 days at 20°C and their subsequent germination compared with that of untreated seed. Four replications of 50 seeds for germination and 25 seeds for emergence were arranged in a 2-factor factorial completely randomised design. All...

Either NO(3) (-) (16 millimolar) or NH(4) (+) (1 millimolar) completely inhibited infection and nodulation of white clover seedlings (Trifoliin repens) inoculated with Rhizobium trifolii. The binding of R. trifolii to root hairs and the immunologically detectable levels of the plant lectin, trifoliin, on the root hair surface had parallel declining slopes as the concentration of either NO(3) (-...