BACKGROUND The water-soluble extract of Ganoderma lucidum (Reishi) has been used as an immunomodulator to stimulate spleen cells proliferation and cytokine expression. OBJECTIVES To investigate the effect of Ganoderma lucidum (G. lucidum) on cytokine production by mice peritoneal macrophages. METHODS Mice peritoneal macrophages were prepared by intra-peritoneal injection of 5 ml cold PBS. P...

Tissue macrophages play a crucial role in the maintenance of tissue homeostasis and also contribute to inflammatory and reparatory responses during pathogenic infection and tissue injury. The high heterogeneity of these macrophages is consistent with their adaptation to distinct tissue environments and specialization to develop niche-specific functions. Although peritoneal macrophages are one o...

In vitro, macrophages from normal strain BRVR mice killed Salmonella more quickly than did macrophages from normal strain BSVS mice. Salmonella injected intraperitoneally multiplied more quickly in BSVS mice than in BRVR mice. BRVR macrophages injected intraperitoneally into BSVS mice protected against Salmonella multiplication better than did BSVS macrophages. The populations of peritoneal cel...

The glucan that was produced by glucosyltransferases (GTFs) from Streptococcus mutans was examined for its stimulating functions toward murine peritoneal macrophages. Soluble glucan was obtained by the reaction with cell-free crude GTFs and sucrose, followed by ethanol precipitation, dispersion in water and re-precipitation by ethanol. Soluble glucan, those average molecular weight was about 3 ...

background: cutaneous leishmaniasis (cl) is endemic in many tropical and subtropical regions of the world. due to the prolonged duration of therapy, adverse effect and resistance to current drugs in the treatment of cl, the dis­covery of novel, efficient, and safe leishmanicidal drugs is required. the aims of the present study was to synthesis of new compounds based on the active compounds of 5...

Atheromatous and xanthomatous lesions contain macrophages laden with lipid, especially cholesteryl esters (1-4). The study of the cholesterol and lipoprotein metabolism of these macrophages has not been extensively carried out because isolation and culture of these cells is difficult. Other sources of macrophages, including rodent peritoneal macrophages, have been investigated with particular e...

Acquired cellular immunity to infection with Mycobacterium tuberculosis is believed to reside in the capacity of mononuclear phagocytes of immunized animals to inhibit intracellular multiplication of the parasite. However, in macrophage tissue culture systems, it has been customary to employ streptomycin in the medium for the purpose of restricting extracellular, but not intracellular, growth o...

Chronic inflammation is associated with an increased number of leukocytes in the spleen, which are then redirected to the site of inflammation. However, it remains unknown how leukocyte recruitment is regulated. Herein, chronic inflammation was induced by intraperitoneal injection of pristane into mice. Leukocytes in the spleen or in the peritoneal cavity were quantified by flow cytometry. We f...

Activation of cannabinoid receptor 2 (CB2R) ameliorates inflammation, but the underlying mechanism remains unclear. In the present study, we examined whether activation of CB2R could suppress the nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) inflammasome. In peritoneal macrophages isolated from C57BL/6 mice, LPS/DSS challenge for 24 h increased the expression of the compon...

Macrophages limit inflammatory responses by clearing apoptotic cells. Deficiencies in apoptotic cell phagocytosis have been linked to autoimmunity. In this study, we determined the efficiency with which macrophages from diabetes-prone NOD and diabetes-resistant NOR, Idd5, Balb/c, and C57BL/6 mice phagocytose apoptotic thymocytes and NIT-1 insulinoma cells. Peritoneal and bone marrow-derived mac...