نتایج جستجو برای: peripheral blood mononuclear cell
تعداد نتایج: 2306671 فیلتر نتایج به سال:
BACKGROUND Recent research has demonstrated that circulating peripheral blood mononuclear fractions (PBMC) containing haematopoietic stem (HSC)/progenitor cells have the potential to play a crucial role in regenerative medicine strategies. Work in our laboratory has shown that a peripheral blood mononuclear cell fraction (PBMC) enhances cartilage repair in an osteochondral defect model in sheep...
Leptospira interrogans glycolipoprotein (GLP) has been implicated in pathological and functional derangement seen in leptospirosis. The goal of this study was to evaluate GLP's ability to induce cellular activation, as assessed by cytokine production and expression of surface activation markers. GLP extracted from either pathogenic L. interrogans serovar Copenhageni or nonpathogenic Leptospira ...
Single cell trapping is demonstrated on superhydrophobic–superhydrophilic patterned microarrays. Superhydrophilic spots that are twice the size of cells to be trapped found optimal for obtaining single trapping. The based exclusivity instead heavy dilution suspension and relying Poisson statistics. superhydrophobic areas array very resistant toward unwanted adhesion thus cleaning steps not need...
OBJECTIVE To evaluate idiopathic hirsutism etiology via molecular studies testing peripheral and local aromatase and 5α-reductase expression. DESIGN Assessment of the expression of messenger RNA (mRNA) for type 1 and 2,5α-reductase isoenzyme gene (SDR5A1, SDR5A2) and aromatase (CYP19A) in dermal papillae cells and peripheral blood mononuclear cells. SETTING University hospital. PATIENT(S)...
patients and methods in this cross-sectional study, peripheral mononuclear cells (pbmcs) were isolated from peripheral blood of 29 patients with newly diagnosed pulmonary tb and 19 patients with positive tuberculin skin test. the pbmcs were activated with ppd for 72 hours. activated cells were harvested, rna was extracted and cdna was synthesized. a real-time taqman method was designed and opti...
A better understanding of the mechanism(s) of cell mediated toxicity for colon cells in vitro may help clarify the pathogenesis of inflammatory bowel disease (IBD). We have examined both the cytotoxicity of IBD peripheral blood mononuclear cells and the kinetics of induction of such toxicity by soluble plasma factors. Peripheral blood mononuclear cells from IBD patients were found to be cytotox...
PROBLEM How maternal allergy affects the systemic and local immunological environment during pregnancy and the immune development of the offspring is unclear. METHOD OF STUDY Expression of 40 genes was quantified by PCR arrays in placenta, peripheral blood mononuclear cells (PBMC), and cord blood mononuclear cells (CBMC) from 7 allergic and 12 non-allergic women and their offspring. RESULTS...
OBJECTIVE To perform systematic transcriptomic analysis of multiple sclerosis (MS) risk genes in peripheral blood mononuclear cells (PBMCs) of subjects with distinct MS stages and describe the pathways characterized by dysregulated gene expressions. METHODS We monitored gene expression levels in PBMCs from 3 independent cohorts for a total of 297 cases (including clinically isolated syndromes...
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