The transepithelial chloride permeability of airway and sweat ductal epithelium has been reported to be decreased in patients with cystic fibrosis (CF). In the present study, we investigated whether the airway epithelial defect was in the cell path by characterizing the relative ion permeabilities of the apical membrane of respiratory epithelial cells from CF and normal subjects. Membrane elect...

Background/Aim. During peritoneal dialysis (PD) an exchange of substances between blood and dialysate takes place through specific histological structures of peritoneum. Peritoneal double-layered serous membrane has, so far, mostly been studied with electron microscopy on experimental animals. The aim of this study was to assess integrity of peritoneal tissue in end-stage renal disease (ESRD) a...

Glutamate transporters play a key role in glutamate clearance and protect the central nervous system from glutamate excitotoxicity. Dysfunctional glutamate transporters contribute to the pathogenesis of Parkinson's disease (PD); however, the mechanisms that underlie the regulation of glutamate transporters in PD are still not well characterized. Here we report that Nedd4-2 mediates the ubiquiti...

During peritoneal dialysis (PD), the peritoneum is exposed to bioincompatible dialysis fluids, which causes progressive fibrosis and angiogenesis and, ultimately, ultrafiltration failure. In addition, repeated episodes of peritonitis or hemoperitoneum may accelerate all these processes. Fibrosis has been classically considered the main cause of peritoneal membrane functional decline. However, i...

Acyl-coenzyme A:cholesterol acyltransferase (ACAT) catalyzes the formation of cholesterol esters (CE) from free cholesterol and fatty acyl-coenzyme A. This experiment was conducted to study the ontogeny of ACAT activity in the yolk sac membrane, liver, and intestine during embryonic development and early posthatch growth of turkeys. The ACAT activity was measured on tissue samples collected at ...

High-performance platinum group metal-free (PGM-free) electrocatalysts were prepared from porous organic polymers (POPs) precursors with highly-porous structures and adjustable surface area. A resin phenol-melamine-based POP an iron salt used to synthesize Fe−N−C catalysts different contents (0.2–1.3 wt.%). Electrochemical spectroscopical characterization allowed us elucidate the effect of Fe c...

Immune checkpoint blockade (ICB) therapies, such as immune inhibitors against programmed death ligand-1 (PD-L1), have not been successful in treating patients with pancreatic ductal adenocarcinoma (PDAC). Despite the critical role of PD-L1 various types cancers, regulatory mechanism expression on cell surface PDAC is poorly understood. Therefore, uncovering potential modulators localisation may...

Before discussing clinical application and its complications, it is extremely important to have an understanding of the relevant peritoneal anatomy and physiology. In essence PD involves the transport of solutes and water across a ‘membrane’ that separates two fluid containing compartments: 1. Blood in peritoneal capillaries, which contains uremic toxins 2. Dialysis solution in the peritoneal c...

The purpose of the article is to reveal role thickness layer lead-palladium alloy deposited on a copper-palladium membrane in processes cathodic injection and anodic extraction atomic hydrogen.The objects study were ~ 4 μm thick film electrodes obtained by magnetron sputtering target with composition 56 at. % Cu 44 Pd. studies carried out cyclic voltammetry double step anodic-cathodic chronoamp...

In artificial lipid bilayer membranes, the ratio of the water permeability coefficient (Pd(water)) to the permeability coefficient of an arbitrary nonelectrolyte such as n-butyramide (Pd(n-butyramide)) remains relatively constant with changes in lipid composition and temperature, even though the individual Pd's increase more than 100-fold. I propose that this is a general rule that also holds ...