CD83 is a marker of dendritic cell (DC) differentiation/activation and its expression in the mouse thymus contributes to CD4(+) T lymphocyte development. Its extrathymic role remains unclear despite the functional effects observed with CD83 fusion proteins or CD83 antibody and recent reports of potential ligands. We investigated the previously observed and presumed functional blockade of the al...

We investigate the analytical representation of potentials of mean force (pmf) using the Morse/long-range (MLR) potential approach. The MLR method had previously been used to represent potential energy surfaces, and we assess its validity for representing free-energies. The advantage of the approach is that the potential of mean force data only needs to be calculated in the short to medium rang...

Locomotor modulation of disynaptic EPSPs from the mesencephalic locomotor region in cat motoneurons. J. Neurophysiol. 80: 3284-3296, 1998. When low-frequency tetanization of the mesencephalic locomotor region (MLR) produce fictive locomotion in unanesthetized, decerebrate cats, each MLR stimulus produces a distinctive cord dorsum potential (CDP) and oligosynaptic excitatory postsynaptic potenti...

The antiviral drugs that inhibit human immunodeficiency virus (HIV) entry to the target cells are already in different phases of clinical trials. They prevent viral entry and have a highly specific mechanism of action with a low toxicity profile. Few QSAR studies have been performed on this group of inhibitors. This study was performed to develop a quantitative structure-activity relationship (...

The auditory middle latency response (MLR), a useful tool in the assessment of low-frequency auditory sensitivity, can be consistently recorded in young children during wakefulness, stages 1 and 2, and REM sleep. Responses are often absent or questionable during stage 4. An on-line measure indicating favorable periods for recording MLR during sleep is important for interpretation of absent pote...

Although the production of B cell stimulatory factors by cell lines and hybridomas is well established, production of specific lymphokines by normal T cells in response to antigen stimulation has not been analyzed. We have used bioassays and neutralizing mAbs to demonstrate that IL-2, IL-4, and B cell growth factors (BCGF) are produced during primary and secondary MLRs. IL-2 is detected in the ...

This chapter summarizes experiments which were designed to reveal how repetitive electrical stimulation of the mesencephalic locomotor region (MLR) recruits nearby hindbrain neurons into activity, such that locomotion can ensue in the tiger salamander, A. tigrinum. The MLR stimulus strength was subthreshold or near-threshold for locomotor movements to ensue. Such relatively weak stimulation of ...

Twenty-six unexplained secondary recurrent aborters underwent vaccinations by using their husband's mononuclear leukocytes according to the protocol adopted for primary recurrent aborters (group I). No mixed lymphocyte culture reaction-blocking antibodies (MLR-BAbs) detected by one-way MLR between spouses were observed in these patients prior to vaccination. In all 26 patients, MLR-BAbs appeare...

Effects of anti-IL2 receptor antibody (anti-IL2 RAb) and cyclosporin A (CsA) on human T cell proliferation in primary mixed lymphocyte reaction (MLR) were investigated. Both agents inhibited the proliferative response induced by alloantigen in a dose dependent manner when they were added at the initiation of culture. We also analyzed the expression of activation antigen on responder cells and t...

Quantitative structure-activity relationship (QSAR) models were employed for prediction the activity of P2X7 receptor antagonists. A data set consisted of 50 purine derivatives was utilized in the model construction where 40 and 10 of these compounds were in the training and test sets respectively. A suitable group of calculated molecular descriptors was selected by employing stepwise multiple ...