This work reports a high throughput and label-free microfluidic cell deformability sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). The sensor relies on differentiating the RBC deformability (a mechanical biomarker) that is highly correlated with the infection status. The cell deformability is measured by eval...

Background: In experiments with homeopathic medicines is important to test the inert vehicle from succussed preparations for treatment control. Aim: To evaluate effect of hydro-alcoholic solutions 1cH, 6cH and 30cH in mice’s experimental infection Trypanosoma cruzi. Methodology: a blind, randomized, controlled test, two independent 34 51 swiss male mice, 8 weeks old, kept cages micro acclimat...

BACKGROUND Plasmodium knowlesi commonly causes severe malaria in Malaysian Borneo, with high case-fatality rates reported. We compared risk, spectrum, and outcome of severe disease from P. knowlesi, Plasmodium falciparum, and Plasmodium vivax and outcomes following introduction of protocols for early referral and intravenous artesunate for all severe malaria. METHODS From September 2010 to Oc...

Plasmodium vivax can cause severe malaria, however its pathogenesis is poorly understood. In contrast to P. falciparum, circulating vivax parasitemia is low, with minimal apparent sequestration in endothelium-lined microvasculature, and pathogenesis thought unrelated to parasite biomass. However, the relationships between vivax disease-severity and total parasite biomass, endothelial autocrine ...

Malaria is a parasitic infectious disease that remains one of the focuses world health problems. The ethanol extract Hedyotis corymbosa has biochemical compounds potentially become new antimalarial drug. This study aimed to analyze effect this on parasitemia and hepatomegaly in mice infected with Plasmodium berghei ANKA. Twentyfive BALB/c were P. ANKA grouped into 5 groups. Group 1-3 treated 25...

The traditional light microscopy has limitations for precise growth assays of malaria parasites in culture or for assessment of new compounds for antimalarial activity; the speed and high reproducibility of flow cytometry can overcome these limitations. A flow cytometric method using PicoGreen, a DNA-binding fluorochrome, was developed with optimal precision suitable for performing growth assay...

Adult residents of holoendemic malaria regions in Africa have a naturally acquired immunity (NAI) to malaria that renders them more resistant to new infections, limits parasitemia, and reduces the frequency and severity of illness. Given such attributes, it is not clear how one might evaluate drug or vaccine efficacy in adults without serious confounding. To determine symptomatic and asymptomat...