A variety of group 4 metal catalytic systems (C2-symmetric {EBTHI}-, {SBI}-type zirconocene complexes (C2-1–4); C1-symmetric (C1-5–8) and Cs-symmetric (Cs-9) {Cp/Flu}-type zirconocene complexes; Cp*2ZrCl2 (Cp* 2-10)), half-metallocene complexes (CpTiCl3, HM-11), constrained-geometry (CGC-12) titanium catalysts) and post-metallocene catalysts (Dow’s ortho-metallated amido-pyridino hafnium comple...

To investigate the mechanisms regulating polarized vesicle delivery to the cell surface in hepatocytes, we have characterized the endogenous plasma membrane (PM)-associated syntaxins. These integral membrane proteins are components of the membrane docking/fusion apparatus and are thought to function as vesicle receptors at the PM. In hepatocytes, the PM is divided into two domains, the apical a...

The authors investigated the phenomenon that performance in an ongoing task declines when individuals must carry out a prospective memory (PM) task. This effect is referred to as the PM interference effect. The authors examined whether the PM interference effect differs between event-based and time-based PM tasks and whether it is increased among the elderly. The authors also investigated adult...

Endoplasmic reticulum (ER)-plasma membrane (PM) junctions are highly conserved subcellular structures. Despite their importance in Ca(2+) signaling and lipid trafficking, the molecular mechanisms underlying the regulation and functions of ER-PM junctions remain unclear. By developing a genetically encoded marker that selectively monitors ER-PM junctions, we found that the connection between ER ...

The study analyzes Polygonal Mirror (PM) scanning heads for one of their most utilized setups: with an off-axis PM and the incident parallel laser beam orthogonal to the optical axis of the objective lens. The study places under the umbrella of the same theory systems that range from PMs with a small number of facets (e.g., industrial measurements) to PMs with a large number of facets as for hi...

Many signaling, cytoskeletal, and transport proteins have to be localized to the plasma membrane (PM) in order to carry out their function. We surveyed PM-targeting mechanisms by imaging the subcellular localization of 125 fluorescent protein-conjugated Ras, Rab, Arf, and Rho proteins. Out of 48 proteins that were PM-localized, 37 contained clusters of positively charged amino acids. To test wh...

Cells employ regulated transport mechanisms to ensure that their plasma membranes (PMs) are optimally supplied with cholesterol derived from uptake of low-density lipoproteins (LDL) and synthesis. To date, all inhibitors of cholesterol transport block steps in lysosomes, limiting our understanding of post-lysosomal transport steps. Here, we establish the cholesterol-binding domain 4 of anthroly...

Based on Cuppen’s divide-and-conquer method [4] and bisection and multisection with the Sturm sequence evaluation coupled with inverse iteration [12, 8, 1], numerous parallel algorithms for solving the eigenvalue problem of real symmetric tridiagonal matrices Tn are presented in the literature [5, 3, 10, 7]. While Cuppen’s divide-and-conquer method achieves the better accuracy results in terms ...

Lethal giant larvae (Lgl) plays essential and conserved functions in regulating both cell polarity and tumorigenesis in Drosophila melanogaster and vertebrates. It is well recognized that plasma membrane (PM) or cell cortex localization is crucial for Lgl function in vivo, but its membrane-targeting mechanisms remain poorly understood. Here, we discovered that hypoxia acutely and reversibly inh...