The detection of proliferating cell nuclear antigen (PCNA), an endogenous cell replication marker, has lacked sensitivity in paraffin-embedded archival tissues fixed in formalin. An enhanced immunohistochemical procedure to detect PCNA has been successfully applied to rat and mouse tissues. Tissue sections are heated in a microwave oven in the presence of an antigen-retrieval solution of heavy-...

The extraction and amplification of nucleic acid from formalin-fixed and paraffin-embedded tissues has become an important exercise in the collection of retrospective epidemiological data. A protocol is described that enables the extraction and amplification of dsDNA from fixed tissues within paraffin blocks and from specimens stored in 10% (aq) formalin. The procedure can be used for the exami...

The implementation of next-generation sequencing (NGS) in clinical oncology has enabled the analysis multiple cancer-associated genes for diagnostics and treatment purposes. detection pathogenic likely mutations is crucial to manage disease. Obtaining mutational profile may be challenging samples with low yields DNA—reflected by type biological material, such as formalin-fixed paraffin-embedded...

Cell suspensions were prepared from fresh and paraffin embedded samples of lymph nodes from nine patients with non-Hodgkin's lymphoma. DNA flow cytometry was performed on these samples and the results from fresh tissue compared with those from paraffin embedded material. Results were compared in terms of DNA index (as a measure of aneuploidy) and S phase fraction (as an indication of proliferat...

Markers of cell proliferation are widely used as diagnostic and prognostic tools. Coincident estimation these markers increases the precise evaluation proliferative status different tissues can also be helpful in determining progression, aggressiveness prognosis lesions. The current study investigated expression PCNA MCM3 40 formalin fixed paraffin embedded tissue blocks odontogenic keratocyst ...

Background and objective: Different molecular alterations have been described in the pathogenesis of endometrial carcinoma, among themis mutation PTEN (phosphatase tension homolog), a tumor suppressor gene. This study aimed to detect immunoexpression carcinoma asses association between with clinicopathological parameters. Methods: cross-sectional retrospective was carried out January 2016-Decem...

BACKGROUND The previous studies showed that herpes human virus-6 (HHV-6) and HHV-7 exist in salivary glands. One of the important areas in oral and maxillofacial pathology field is tumors of the salivary glands. In this study, to declare the major sites of persistent infection with HHV-6 and HHV-7, the existence of HHV-6 and HHV-7 genomes in formalin-fixed paraffin embedded tissue samples of sa...