UNLABELLED Chondrocyte dedifferentiation presents a major barrier in engineering functional cartilage constructs. To mitigate the effects of dedifferentiation, this study employed a post-expansion aggregate culture step to enhance the chondrogenic phenotype of passaged articular chondrocytes (ACs) before their integration into self-assembled neocartilage constructs. The objective was twofold: (...

Previous QTL mapping identified a Brassica nigra homolog to Arabidopsis thaliana CO as a candidate gene affecting flowering time in B. nigra. Transformation of an A. thaliana co mutant with two different alleles of the B. nigra CO (Bni COa) homolog, one from an early-flowering B. nigra plant and one from a late one, did not show any differential effect of the two alleles on flowering time. The ...

This study aims to determine the effect of KNO3, H2SO4, and GA3 in breaking dormancy germination salak (Salacca zalacca) plants. In this using a non-factorial randomized block design with one factor, namely P0 (control), P1 (KNO3 60%), P2 70%), P3 80%), P4 (H2SO4 P5 P6 P7 (GA3 25 ppm), P8 50 P9 75 P10 60% P11 70% P12 80% P13 P14 P15 75). Parameters observed were germination, maximum growth pote...

Present investigations were carried out in tomato assess the mean performance, general and range for nineteen quantitative characters. Forty-five hybrids generated by crossing ten parents randomized complete block design with three replications at Main Experiment Station, Department of Vegetable Science, ANDUA&T, Kumarganj, Ayodhya, during winter (Rabi) season 2020-2021 2021-2022. The poole...

The floral regulators GIGANTEA (GI), CONSTANS (CO), and FLOWERING LOCUS T (FT) play key roles in the photoperiodic flowering responses of the long-day plant Arabidopsis thaliana. The GI-CO-FT pathway is highly conserved in plants. Here, we demonstrate that the circadian clock proteins LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK-ASSOCIATED1 (CCA1) not only repressed the floral transition ...

AIM The aim of this study was to evaluate parents' perceptions of dental fluorosis and other oral health conditions depicted in photographs of anterior teeth. MATERIALS AND METHODS The sample comprised 120 parents (average age 39.3 years) whose children were undergoing dental treatment at the School of Dentistry of the Federal University of Minas Gerais. Seven photographs were selected: P1, o...

Studies on the regulation of phage Ø29 gene expression revealed a new mechanism to accomplish simultaneous activation and repression of transcription leading to orderly gene expression. Two phage-encoded early proteins, p4 and p6, bind synergistically to DNA, modifying the topology of the sequences encompassing early promoters A2c and A2b and late promoter A3 in a hairpin that allows the switch...

Allopolyploidy is formed by combining two or more divergent genomes and occurs throughout the evolutionary history of many plants and some animals. Transcriptome analysis indicates that many genes in various biological pathways, including flowering time, are expressed nonadditively (different from the midparent value). However, the mechanisms for nonadditive gene regulation in a biological path...