Pseudomonas aeruginosa RocR, an EAL-domain protein which regulates the expression of virulence genes and biofilm formation, has been cloned and expressed in Escherichia coli and purified. Here, the crystallization and preliminary diffraction analysis of RocR are reported. The X-ray diffraction data were processed to a resolution of 2.50 A. The crystals belonged to space group P6(1)22 or P6(5)22...

All transcripts of the human parvovirus B19 identified so far are regulated by a single promoter at map unit 6 of the viral genome, the so-called p6 promoter. This promoter is active in a wide variety of different cells. In order to identify cellular transcription factors involved in regulating promoter activity, we performed gel-retardation and supershift assays using the parts of the p6 promo...

Purpose Acupuncture has gained popularity since the National Institutes of Health 1997 Consensus Statement concluded that acupuncture is an effective anti-emetic for adult postoperative and chemotherapy-induced nausea and vomiting. The most commonly used acupuncture point to control nausea and vomiting is the Pericardium 6 (P6), or Neiguan point. Some evidence suggests that the anti-emetic effe...

In Figure 1, the set S consists of thirteen points. The output of a convex hull algorithm should be the list (p1, p2, p3, p4, p5, p6). We remark that the list storing the vertices of CH (S) can start with an arbitrary vertex. In the example, the list (p3, p4, p5, p6, p1, p2) would also be a valid output. ∗School of Computer Science, Carleton University, Ottawa, Ontario, Canada K1S 5B6. E-mail: ...

Introduction p1 1 Background p2 a) The EKV MOS Model Formulation and Usage p2 b) The Switched Current Memory Cell Principle p4 2 Tips on selecting simulator parameters for a discrete-time simulation p6 3 Example 1Regulated Cascode Memory Cell (saturated) p6 4 Example 2 SI track-and-hold cell, weak/moderate inversion p8 5 Issues on higher-level modeling p11 6 Conclusions p11 7 References p11

Haemophilus influenzae type b Minn A produced 12 lipoproteins with apparent molecular weights of between 14,000 and 67,000. The lipoproteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography of delipidated extracts of cells grown in [3H]palmitate. When the delipidated cell extracts were subjected to acid methanolysis, tritium was quantitatively recove...

A graph is H-free if it does not contain an induced subgraph isomorphic to H . We denote by Pk and Ck the path and the cycle on k vertices, respectively. In this paper, we prove that 4-COLORING is NP-complete for P7-free graphs, and that 5-COLORING is NP-complete for P6-free graphs. These two results improve all previous results on kcoloring Pt-free graphs, and almost complete the classificatio...

HIV-1 and other retroviruses exit infected cells by budding from the plasma membrane, a process requiring membrane fission. The primary late assembly (L) domain in the p6 region of HIV-1 Gag mediates the detachment of the virion by recruiting host Tsg101, a component of the class E vacuolar protein sorting (Vps) machinery. We now show that HIV Gag p6 contains a second region involved in L domai...