adsorption of 1-butanol, phenol, and p-nitrophenol on activated carbon havebeen studied. the results were used to testifying the validity of different modelsbased on thermodynamic principles: freundlich, langmuir and henry isotherms.the maximum capacity of adsorbent for three compounds was determined, theconstants of equilibrium were determined by freundlich isotherm and virialisotherm expressi...

OBJECTIVE To investigate the performance of soil-slurry bioreactor used for remediating contaminated soil with 4-nitrophenol (4-NP). METHODS The slurry bioreactor was used to degrade different concentrations of 4-nitrophenol with or without inoculating the acclimated activated sludge. HPLC system (Hewlett-Packard model 5050 with a UV detector) was used for the quantification of 4-nitrophenol....

This research evaluates the efficiency of nanosized ZnO in the catalytic ozonation of 4-chloro-2-nitrophenol and determines the effect of pH on heterogeneous catalytic ozonation. The combined use of ozone and ZnO catalyst leads to conversion of 98% 4-chloro-2-nitrophenol during 5 min. In addition, it was found that in ZnO catalytic ozonation, the degradation efficiency of 4-chloro-2-nitrophenol...

We describe specifications for high-purity 4-nitrophenol, which is suitable for spectrophotometric standardization. Such a reference material is needed in clinical enzymology to establish the proper molar absorptivity of 4-nitrophenol under final reaction conditions, particularly for measuring alkaline phosphatase activity in human serum. Some lots of 4-nitrophenol available commercially met th...

A sequencing batch reactor (SBR) was inoculated with p-nitrophenol-degrading activated sludge to biodegrade a mixture of monosubstituted phenols: p-nitrophenol (PNP), PNP and o-cresol; and PNP, o-cresol and o-chlorophenol. Settling times were progressively decreased to promote biomass granulation. PNP was completely biodegraded. The PNP and o-cresol mixture was also biodegraded although some tr...

A primary goal of protein engineering is to control catalytic activity. Here we show that through mutagenesis of three active site residues, the catalytic activity of a multicomponent monooxygenase is altered so that it hydroxylates all three positions of toluene as well as both positions of naphthalene. Hence, for the first time, an enzyme has been engineered so that its regiospecific oxidatio...

BACKGROUND: The aim of our investigation was to estimate the stability of alpha-tocopheryl O-glycosides in relation to activity of exoglycosidases in selected rat tissues. MATERIAL AND METHODS: Acetylated glycosides were obtained in glucosidation of alpha-tocopherol using the Helferich method. The activity of exoglycosidases was determined by the Zwierz et al. method. Protein concentrations wer...

In the present study, photocatalytic removal of p-nitrophenol has been carried out in the presence of ZnO by using a batch reactor with a UV-C lamp (15 W). The effects of applied ZnO concentration and UV light intensity have been studied. The increase of initial pollutant concentration decreases the removal rate, and the results indicate that the apparent reaction rate constant in the UV/Zn...

The first complete genome sequence of a p-nitrophenol (PNP)-degrading bacterium is reported here. Pseudomonas putida DLL-E4, a Gram-negative bacterium isolated from methyl-parathion-polluted soil, can utilize PNP as the sole carbon and nitrogen source. P. putida DLL-E4 has a 6,484,062 bp circular chromosome that contains 5,894 genes, with a G+C content of 62.46%.

0-0005 to 0-004M resulted in progressive acceleration of respiration. Glucose plus hexokinase accelerated respiration, which was further increased by AMP. 3. The addition to the mitochondrial fraction of the particle-free supernatant of kidney cortex greatly enhanced the stimulating action of p. nitrophenol on the respiration. The supernatant fraction of mammary tissue did not show a correspond...