Oxytetracycline (OTC) is administered in high doses to livestocks and enters the environmental compartments as a consequence of animal waste disposal. As a first step in setting up a useful mycoremediation technique, an OTC lab degradation test was performed in liquid medium using the ligninolytic fungus Pleurotus ostreatus. OTC disappearance in culture medium was clearly evident as early as th...

Individual lines of Vitis vinifera Barbera and Chardonnay were obtained by in vitro culture of axillary buds collected from phytoplasma-affected mother plants. The molecular assays did not detect the “flavescence dorée” (FD) phytoplasma in the 66 lines tested after 6-9 months of culture, while the “bois noir”-associated phytoplasma was detected in 22 out of 50 lines. Despite the observed ineffi...

Physiological studies have shown that Streptomyces rimosus produces the polyketide antibiotic oxytetracycline abundantly when its mycelial growth is limited by phosphate starvation. We show here that transcripts originating from the promoter for one of the biosynthetic genes, otcC (encoding anhydrotetracycline oxygenase), and from a promoter for the divergent otcX genes peak in abundance at the...

Onchocerca ochengi, a filarial parasite of cattle, represents the closest relative of the human pathogen, Onchocerca volvulus. Both species harbour Wolbachia endosymbionts and are remarkable in that adult female worms remain viable but sessile for many years while surrounded by host cells and antibodies. The basis of the symbiosis between filariae and Wolbachia is thought to be metabolic, altho...

Inhibition of the proliferation of Ehrlichia risticii cultured in murine macrophage P388D1 cells by eight antibiotics was evaluated by indirect fluorescent-antibody staining with an antiserum specific to E. risticii. There was a negative correlation between the percentage of infected cells and the log10 of the concentrations of all antibiotics examined. The ranks of the antibiotics in the order...

The tissue distribution of a long-acting oxytetracycline formulation after intramuscular administration to calves at a dose rate of 20 mg/kg was studied. Oxytetracycline concentrations were determined in lung, bone marrow, mammary gland, uterus, uterine horn, ovary, liver, synovial fluid, joint tissue, kidney, spleen, brain, muscle, fat, urine, bile, saliva, ruminal content and serum. Observed ...

The anodic oxidation of oxytetracycline was performed with success using as anode a boron-doped diamond electrode. The experiments were conducted in batch mode, using two different electrochemical cells: an up-flow cell, with recirculation, that was used to evaluate the influence of recirculation flow rate; and a stirred cell, used to determine the influence of the applied current density. Besi...

An Ehrlichia ruminantium culture system was utilized for the anti-rickettsial evaluation of two ethnoveterinary plants, Elephantorrhiza elephantina and Aloe marlothii. Well-established E. ruminantium cultures were incubated with the plant leaf acetone extracts and compared to oxytetracycline and untreated controls. Effectivity was established by comparing the percentage parasitised cells and th...

INTRODUCTION Antibiotics are largely employed in zootechnical feed to preserve human and animal species from zoonosis due pathogenic infective agents. AIM Due to the increasing number of pathologies related to diet (e.g. food intolerances), we investigated the toxic effects induced by antibiotics residues, oxytetracyclines, present within the industrial food on both human and domestic animals...