The over-expression of basic fibroblast growth factor (bFGF) plays a crucial role in the development, invasion and metastasis of lung cancer. Therefore, neutralizing antibodies against bFGF may inhibit the growth of lung cancer. In this study, a Disulfide-stabilized diabody (ds-Diabody) against bFGF was constructed by site-directed mutation and overlap extension PCR (SOE-PCR) at the position of...

BACKGROUND During the development of a therapeutic antibody, large numbers of monoclonal antibodies are required to screen for those that are best suited for the desired activity. Although the single cell-based immunoglobulin variable gene cloning technique is a powerful tool, the current methods remain an obstacle to the rapid production of large numbers of recombinant antibodies. RESULTS We...

BACKGROUND The development of infections with ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) remains a serious problem in recipients of stem cell or organ transplants. Nearly all GCV-resistant clinical isolates have mutations in the viral UL97 gene. The rapid detection of GCV-resistant HCMV infections is necessary and the relative proportions of wild-type and mutant strains are predic...

A novel method for site-directed mutagenesis of DNA sequences based on the use of the PCR is described. The method uses two oligonucleotide primers that contain the desired sequence change and overlap at their 5' ends. In addition, the thymine residues in the overlap region have been substituted with deoxyuracil. Amplification of the template plasmid by PCR results in incorporation of the prime...

This paper describes a SlipChip to perform digital PCR in a very simple and inexpensive format. The fluidic path for introducing the sample combined with the PCR mixture was formed using elongated wells in the two plates of the SlipChip designed to overlap during sample loading. This fluidic path was broken up by simple slipping of the two plates that removed the overlap among wells and brought...

Part 1. Chains as pseudo–orbits 8 2. Pesin charts 8 2.1. Non-uniform hyperbolicity 8 2.2. Lyapunov change of coordinates 8 2.3. Pesin Charts 10 2.4. Distortion compensating bounds 11 2.5. NUHχ (f) 11 3. Overlapping charts 11 3.1. The overlap condition 12 3.2. The form of f in overlapping charts 14 3.3. Coarse graining 16 4. ε–chains and an infinite-to-one Markov extension of f 18 4.1. Double ch...

Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are severe adverse cutaneous reactions to drugs. We describe the case of a 19 year old patient with SJS/TEN overlap syndrome, who developed severe interstitial pneumonia after she had received antiepileptic drugs. A cytomegalovirus infection was diagnosed by Real Time Polymerase Chain Reaction (RT-PCR) detection on Bronchoalveo...

In this paper, a new approach that extends narrow-band excitation signals using synchronous overlap and add (SOLA) of spectra have been proposed. Although artificial bandwidth extension (ABE) of speech has been extensively studied, the role of excitation spectra has not been as widely studied as the spectral envelope extension. In this study ABE is investigated with the widely used source-filte...

“megaprimer method” of site-directed mutagenesis. BioTechniques 8:404-407. 11.Senanayake, S.D. and D.A. Brian. 1995. Precise large deletions by the PCR based overlap extention. Mol. Biotechnol. 4:13-15. 12.Silver, J., T. Limjoco, and Feinstone. 1995. Site-specific mutagenesis using the polymerase chain reaction, p. 179-188. In M.A. Innis, D.H. Gelfand and J.J. Sninsky (Eds.), PCR Strategies. Ac...

Polymerase-mediated recombination based on DNA polymerase chain reactions (PCRs) has been used to carry out directed joining at a present point of two DNA fragments initially contained in a plasmid and a single-stranded synthetic DNA. The process includes copying of these fragments by PCR with generation of an overlapping homologous region. Such overlap of 12 base pairs in length was found to b...