Bioprocess scale-up is a fundamental component of process development in the biotechnology industry. When scaling up a mammalian cell culture process, it is important to consider factors such as mixing time, oxygen transfer, and carbon dioxide removal. In this study, cell-free mixing studies were performed in production scale 5,000-L bioreactors to evaluate scale-up issues. Using the current bi...

In this work, extraction of propionic acid from the aqueous phase to the organic phase (1-octanol) was performed in T-junction microchannels and effects of channel diameter and fluid flow rate on the mass transfer characteristics were investigated. The two-phase flow patterns in studied microchannels with 0.4 and 0.8 mm diameters were observed. Weber ‎ number and surface-to-volume ratio were ca...

Microalgal cultures are usually sparged with CO2-enriched air to preclude CO2 limitation during photoautotrophic growth. However, the CO2 vol% specifically required at operating conditions to meet the carbon requirement of algal cells in photobioreactor is never determined and 1-10% v/v CO2-enriched air is arbitrarily used. A scheme is proposed and experimentally validated for Chlorella vulgari...

A compact static mixer for gas–liquid dispersion in pipelines is studied this paper with a Reynolds averaged two fluid model approach. procedure based on the lumped parameter solution of population balance applied to obtain bubble Sauter mean diameter needed interphase forces. The gas distribution pipe analyzed different operative conditions and efficiency assessed terms homogeneity section, lo...

The hydrodynamic characteristics of mixing fluids are always the points to consider in improvement their quality especially using motionless mixers normally stated as “Static Mixers”. Motionless technique was adopted for Air-Water system with advantage negligible power consumption over dynamic mixers. Different were experimented “Baffle Type” static element and compared those already used recen...

Nitrogen fixing cyanobacteria are being increasingly explored for nitrogenase-dependent hydrogen production. Commercial success however will depend on the ability to grow these cultures at high cell densities. Photo-limitation at high cell densities leads to hindered photoautotrophic growth while turbulent conditions, which simulate flashing light effect, can lead to oxygen toxicity to the nitr...

A nanofluid is mixture of nano sized particles and a base fluid. This paper investigates by using laboratory based double pipe heat exchanger model, the performance of nanofluid containing about 48.46nm  particle size nanoparticles (ZnO) without or with addition of nonionic surfactant Rokanol K7 (500ppm) into the base fluid double distilled water to prepared three different concentrations 1.0%,...

The use of organic liquids as vectors to enhance mass transfer has been applied since the 1970s. However, mass transfer in three-phase reactors is only partially understood. This paper aimed to characterize oxygen transfer in three-phase reactors containing air as gas, silicone oil as vector andwater as aqueous phase. Amass transfermodel that considers separately air/vector, vector/water and ai...

The Wave Bioreactor is widely used in cell culture due to the benefits of disposable technology and ease of use. A novel cellbag was developed featuring a frit sparger to increase the system's oxygen transfer. The purpose of this work was to evaluate the sparged cellbag for yeast cultivation. Oxygen mass transfer studies were conducted in simulated culture medium and the sparged system's maximu...