Budding yeast replication origins are activated during S phase according to a predetermined temporal programme. Two recent studies indicate that this programme is executed, at least in part, by the S-phase-promoting cyclins that act to assemble a pre-initiation complex which includes the Cdc45 protein.

Cyclin-dependent kinases (CDKs) drive the cell cycle, central to which is the accurate control of chromosome replication. In Saccharomyces cerevisiae, six closely related B-type cyclins (Clb1-6) drive the events of S phase and mitosis. Either Clb5 or Clb6 can activate early-firing replication origins, whereas only Clb5 can activate late origins. Clb1-4 are expressed later in the cell cycle. Whe...

To prevent re-replication of DNA, the licensing of replication origins is inhibited in S phase and G2. New work by Yamaguchi et al. (in this issue of Cell) shows that the small GTPase Ran can directly inhibit licensing inside nuclei once CDKs are active late in the cell cycle.

Different replication origins in eukaryotes are activated at different times during S phase. New work indicates that the time at which an origin fires is related to its ability to recruit replication initiation factors that are limiting within the cell.

country-of-origin (coo) is one of the most important external cues which influence on the customers purchase decision and has been topic of so many studies. for the same reason, the present article with taking into consideration one hundred forty two students of isfahan university as its statistical sample, first tried to measure the influence of coo on students’ purchase decisions among the sa...

primary retroperitoneal mucinous tumor (prmt) of low malignant potential (border line) is an uncommon neoplasm with fewer than 50 reported cases. uncertain diagnostic imaging results make diagnosis of its origin difficult, preoperatively. later treatment planning and prognosis would be affected by exact diagnosis of the tumor origin. this study presents a case of persian woman with diagnostic, ...

Two dimensional gel electrophoretic techniques were used to locate all functional DNA replication origins in a 22.5 kb stretch of yeast chromosome III. Only one origin was detected, and that origin is located within several hundred bp of an ARS element.