Abstract Study question Can the developmental competence of poor-quality oocytes identified by hyperspectral imaging be restored maternal spindle transfer? Summary answer Maternal transfer can efficiently assisted to identify and subsequently repair oocytes. What is known already Oocyte cytoplasmic dysfunctions (including, but not limited mitochondria) are a major cause for poor embryonic devel...

the fusion of medical images is very useful for clinical application. generally, the pet image indicates the function of tissue and the mri image shows the anatomy of tissue. in this article we fused mri and pet images and the purpose is adding structural information from mri to pet image. the images decomposed with curvelet transform, and then two images fused with applying fusion rules. we us...

Insulin receptor substrate-1 (IRS-1) serves as the major immediate substrate of insulin/insulin-like growth factor (IGF)-1 receptors and following tyrosine phosphorylation binds to specific Src homology-2 (SH2) domain-containing proteins including the p85 subunit of phosphatidylinositol (PI) 3-kinase and GRB2, a molecule believed to link IRS-1 to the Ras pathway. To investigate how these SH2-co...

Intracellular Ca(2+) oscillations at fertilization are responsible for triggering egg activation. The aim of this study was to examine the effect of the age of the oocyte donor and in-vitro maturation on the generation of Ca(2+) transients at fertilization. The results show that <10% of in-vivo and in-vitro matured oocytes from 19-day old mice develop to the blastocyst stage in vitro. In contra...

Cytokeratins have previously been shown to exist in the Xenopus oocyte cortex. Using three monoclonal antibodies against cytokeratins, we follow the appearance of cytokeratin-containing filaments, and their changes in distribution during oocyte differentiation and maturation. Cytokeratin-containing filaments are shown to change dramatically in distribution in the oocyte during its ontogeny.

Prediction and improvement of oocyte competence are two critical issues in assisted reproductive technology to improve infertility therapy. The lack of reliable and objective predictors of oocyte developmental competence for oocyte/embryo selection during in vitro fertilization hampers the effectiveness of this technology. Likewise, the low pregnancy rate resulting from in vitro maturation of h...

Delineation of apical and basolateral membrane domains is a critical step in the epithelialization of the outer layer of cells in the embryo. We have examined the initiation of polarized membrane traffic in Xenopus and show that membrane traffic is not polarized in oocytes but polarized membrane domains appear at first cleavage. The following proteins encoded by injected RNA transcripts were us...

background: it is important to protect oocytes and embryos from oxidative stress in the culture medium. melatonin has been shown to be a direct free radical scavenger. objective: effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. materials and methods: oocytes from supper-ovulated mouse were divided to two groups: cumulus ...

In Drosophila, a single oocyte develops within a 16-cell germline cyst. Although all 16 cells initiate meiosis and undergo premeiotic S phase, only the oocyte retains its meiotic chromosome configuration and remains in the meiotic cycle. The other 15 cells in the cyst enter the endocycle and develop as polyploid nurse cells. A longstanding goal in the field has been to identify factors that are...