Marine hydrocarbon seeps supply oil and gas to microorganisms in sediments and overlying water. We used stable isotope probing (SIP) to identify aerobic bacteria oxidizing gaseous hydrocarbons in surface sediment from the Coal Oil Point seep field located offshore of Santa Barbara, California. After incubating sediment with (13)C-labeled methane, ethane, or propane, we confirmed the incorporati...

A nodule-shaped microbial mat was found subsurface in sediments of a gas seep in the anoxic Black Sea. This mat was dominated by ANME-1 archaea and consumed methane and sulfate simultaneously. We propose that such subsurface mats represent the initial stage of previously investigated microbial reefs.

We have purified DNA from gill tissue of a marine bivalve, Calyptogena soyoae, collected from the deep-sea cold seep communities in Sagami Bay, Japan. An rRNA gene was amplified, cloned, and sequenced. In situ hybridization revealed that the sequence is that of a bacterial endosymbiont within the gill of C. soyoae.

New insight into strati¢cationofanaerobicmethanotrophs in cold seep sediments Irene Roalkvam, Steffen Leth Jørgensen, Yifeng Chen, Runar Stokke, Håkon Dahle, William Peter Hocking, Anders Lanzén, Haflidi Haflidason & Ida Helene Steen Department of Biology, Centre for Geobiology, University of Bergen, Norway; Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, China; and Department...

*Correspondence: Jeffrey J. Marlow and Victoria J. Orphan, Division of Geological and Planetary Sciences, California Institute of Technology, MC 100-23, Pasadena, CA 91101, USA e-mail: [email protected]; [email protected] Methane seeps are among the most productive habitats along continental margins, as anaerobic methane-oxidizing euryarchaeaota and sulfur-metabolizing deltaproteobacte...

Gas seeps emanating from Yanartaş (Chimera), Turkey, have been documented for thousands of years. Active serpentinization produces hydrogen and a range of carbon gases that may provide fuel for life. Here we report a newly discovered, ephemeral fluid seep emanating from a small gas vent at Yanartaş. Fluids and biofilms were sampled at the source and points downstream. We describe site condition...

We used high-resolution imagery within a Geographic Information System (GIS), free gas and porewater analyses animal bulk stable isotope measurements to characterize the biotic abiotic aspects of newly discovered Vestbrona Carbonate Field (VCF) seep site on Norwegian shelf (63°28′N, 6° 31′E, ∿270 m water depth). Free was mainly composed microbial methane. Sediment sulfide concentrations were in...

Carbonate communities: The activity of anaerobic methane oxidizing microbes facilitates precipitation of vast quantities of authigenic carbonate at methane seeps. Here we demonstrate the significant role of carbonate rocks in promoting diversity by providing unique habitat and food resources for macrofaunal assemblages at seeps on the Costa Rica margin (400-1850 m). The attendant fauna is surpr...

Abstract Hydroacoustic surveys at the accretionary wedge southwest of Taiwan reveal a confined active hydrocarbon seepage area ~ 49,000 m 2 in 1350 water depth on northern crest Four-Way Closure Ridge, which we call Yam Seep. In this study, multibeam and side-scan sonar data acquired during with an autonomous underwater vehicle expedition R/V Ocean Researcher I 2017 showed that is characterized...