Four simple and sensitive methods for the assay of prazosin hydrochloride (PRH) are developed. These methods are based on the formation of coloured species by treating it either with excess N-bromosuccinimide (NBS) and determining the unconsumed NBS with p-N-methyl aminophenol sulphate (metol)-sulphanilamide (SA) reagent (method A, lambda(max) 520 nm): with 3-methyl-2-benzothiazolinone hydrazon...

A gas-chromatographic procedure for acetaminophen is described in which the drug is chromatographed as the O-heptyl-N-methyl derivative. This derivative is prepared by a sequential alkylation procedure in which the phenolic hydroxyl group of the parent compound is alkylated off-column with heptyl iodide and the amide group is derivatized on-column by reaction with trimethylanilinium hydroxide. ...

The aim of this work was to demonstrate that interaction of ferric cations with poly(o-aminophenol) (POAP) films causes their deactivation. Spectroelectrochemical measurements and rotating disk electrode voltammetry (RDEV) were employed on both freshly prepared and deactivated POAP films. It was observed a reduction (deactivation) of the redox activity of POAP after its treatment with ferric ca...

INTRODUCTION In this two week experiment you will be synthesising and purifying two common analgesics (painkillers) aspirin and acetaminophen. Aspirin is prepared by the O-acylation (i.e. add an acyl group to an oxygen) of salicylic acid. Similarly, acetaminophen is prepared by the N-acylation (i.e. add an acyl group to a nitrogen) of p-aminophenol. Note the similarity between the two reactions...

Homogenates of six rat tissues metabolized the o-amidofluorenol, N-(1-hydroxy-~fluorenyl)aeetamide, as judged by the disappearanee of the phenolic hydroxyl group of the compound from the ineubation systems. By the same criterion, N-(7-hydroxy-~fluorenyl)aeetamide was resistant to metabolie attack. The deaeylation of N-(1-hydroxy-~-fluorenyl)aeetamide, N-(7-hydroxy-~-fluorenyl)aeetamide, N-s and...

An amperometric glucose biosensor is developed that is based on immobilization of glucose oxidase (GOD) in a composite film of poly(o-aminophenol) (POAP) and carbon nanotubes (CNT), which are electrochemically co-polymerized at a gold (Au) electrode. Because of the high surface per volume ratio and excellent electrical conductivity of CNT, the biosensor based on an Au/POAP/CNT/GOD electrode has...

In this study, zirconium dodecylphosphonate was synthesized by the reported method in scientific literature. 2-Alkylbenzoxazoles were prepared from aliphatic carboxylic acids and 2-aminophenol in the presence of this catalyst under solvent-free conditions at 100°C. Their structures were recognized by IR, 1H NMR, and 13C NMR. Then, we used aromatic carboxylic acids in t...

We have developed an ammonia-sensitive material by coupling the Berthelot reaction to our polymerized crystalline colloidal array (PCCA) technology. The material consists of a periodic array of highly charged colloidal particles (110 nm diameter) embedded in a poly(hydroxyethyl acrylate) hydrogel. The particles have a lattice spacing such that they Bragg-diffract visible light. In the Berthelot...

The predominant bacterial pathway for nitrobenzene (NB) degradation uses an NB nitroreductase and hydroxylaminobenzene (HAB) mutase to form the ring-fission substrate ortho-aminophenol. We tested the hypothesis that constructed strains might accumulate the aminophenols from nitroacetophenones and other nitroaromatic compounds. We constructed a recombinant plasmid carrying NB nitroreductase (nbz...