The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized byreverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coatprotein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibodysandwich enzyme-linked immunesorbent assay (DASELISA) in a population ...

detection of mycoplasma synoviae (ms) by culture and polymerase chain reaction (pcr) has been reported from commercial chicken farms in different provinces of iran. in some reports the phylogenetic analysis of ms isolates based on 16s rrna and variable lipoprotein hemagglutinin (vlha) genes have been carried out. the pcr product containing partial 16s rrna genes of iranain isolates was sequence...

it is difficult to distinguish between clinically significant slowly-growing, non-pigmented mycobacteria, notably to separate m. aviumand m. intracellulare from one another and from m. scrofulaceum strains. the purpose of this study was to evaluate the extent to which 16s rrna sequencing could be used to highlight the taxonomic relationships of the mycobacterial strains, which are difficult to ...

chicken anemia virus was detected by pcr in tissue samples collected from poultry flocks in gujarat,india. the vp1, vp2 and vp3 gene sequences of cav from anand, gujarat were obtained after cloning thepcr products in pdrive cloning vector. nucleotide sequence alignment with other cav sequencesdemonstrated overall identity of 95-98.8%, 98.8-99.8% and 98.8-100% for vp1, vp2 and vp3 regions,respec...

Tobacco mosaic virus (TMV) is one of the devastating plant viruses in the world that infects more than 200 plant species. Movement protein plays a supportive role in the movement of other plant viruses, and viral coat protein is highly expressed in infected plants and affects replication and movements of TMV. In order to investigate genetic variation in the terminal fragment sequence in Iranian...

the pb2 protein of influenza a viruses has been shown as host range determinant. in this study pb2gene of five h9n2 isolates from tehran province during the period of 1998-2001 were partially amplifiedand sequenced to do phylogenetic study. iranian isolates could be classified in eurasian lineage according totheir pb2 gene sequences. the pb2 genes of these isolates are different from those of m...