نتایج جستجو برای: nucleic acid amplification techniques naat

تعداد نتایج: 1401179  

Journal: :Polish journal of pathology : official journal of the Polish Society of Pathologists 2014
Katarzyna Bogusiak Józef Kobos

Human papillomaviruses (HPV) are the etiological factors of many benign and malignant lesions localized in the skin and mucous membranes in the region of the urogenital organs and head and neck. Currently, most assays for the detection of HPV are based on detecting the presence of viral nucleic acids, mostly viral DNA. These molecular techniques can be divided into: 1) methods based on a target...

Journal: :journal of reproduction and infertility 0

chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection, with an estimated annual worldwide incidence of 50 million cases. a large proportion of those infected, particularly women, are asymptomatic, and these individuals serve as a major reservoir of infection. women are also at risk for serious reproductive tract complications with significant morbidity. in an effo...

Journal: :PLoS ONE 2008
Zhen Sun Zhi Chen Xiaoli Hou Shuping Li Haihong Zhu Ji Qian Daru Lu Wei Liu

BACKGROUND Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods. However, the multiplexing method currently used requires precise handiwork and many complicated steps, making a new, simpler technique desirable. Oligonucleotides co...

2005
Robert H. Yolken

Nucleic acid amplification reactions offer a great deal of potential for the accurate and sensitive detectionlquantitation of microbial nucleic acids in human body fluids and have, in some cases, an edge over techniques using immunoassays. Successful detections include : nucleic acids from viruses such as HIV, rotaviruses, coxsackieviruses, papilloma viruses, pestiviruses as well as microorgani...

Journal: :PLoS ONE 2009
Keertan Dheda Richard N. Van-Zyl Smit Leonardo A. Sechi Motasim Badri Richard Meldau Gregory Symons Hoosein Khalfey Igshaan Carr Alice Maredza Rodney Dawson Helen Wainright Andrew Whitelaw Eric D. Bateman Alimuddin Zumla

BACKGROUND Current tools for the diagnosis of tuberculosis pleural effusions are sub-optimal. Data about the value of new diagnostic technologies are limited, particularly, in high burden settings. Preliminary case control studies have identified IFN-gamma-inducible-10 kDa protein (IP-10) as a promising diagnostic marker; however, its diagnostic utility in a day-to-day clinical setting is uncle...

2015
Diana L. Martin Rhiannon Bid Frank Sandi E. Brook Goodhew Patrick A. Massae Augustin Lasway Heiko Philippin William Makupa Sandra Molina Martin J. Holland David C. W. Mabey Chris Drakeley Patrick J. Lammie Anthony W. Solomon

BACKGROUND Trachoma, caused by Chlamydia trachomatis (Ct), is the leading infectious cause of blindness worldwide. Yearly azithromycin mass drug administration (MDA) plays a central role in efforts to eliminate blinding trachoma as a public health problem. Programmatic decision-making is currently based on the prevalence of the clinical sign "trachomatous inflammation-follicular" (TF) in childr...

Journal: :Clinical Laboratory 2021

BACKGROUND: COVID-19 is caused by the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), which was discovered in 2019 and spread around world a short time. SARS-CoV-2 nucleic acid amplification tests (NAATs) have been rapidly developed quickly applied to clinical testing of COVID-19. Aim this study evaluate performance four NAAT assays. METHODS: Limit detection (LOD), precision, accu...

2016
Nicole H. T. M. Dukers-Muijrers Petra F. G. Wolffs Lisanne Eppings Hannelore M. Götz Sylvia M. Bruisten Maarten F. Schim van der Loeff Kevin Janssen Mayk Lucchesi Titia Heijman Birgit H. van Benthem Jan E. van Bergen Servaas A. Morre Jos Herbergs Gerjo Kok Mieke Steenbakkers Arjan A. Hogewoning Henry J. de Vries Christian J. P. A. Hoebe

BACKGROUND In women, anorectal infections with Chlamydia trachomatis (CT) are about as common as genital CT, yet the anorectal site remains largely untested in routine care. Anorectal CT frequently co-occurs with genital CT and may thus often be treated co-incidentally. Nevertheless, post-treatment detection of CT at both anatomic sites has been demonstrated. It is unknown whether anorectal CT ...

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