Simple sequence repeat (SSR markers or microsatellites), based on the specific PCR amplification of DNA sequences, are becoming the markers of choice for molecular characterization of a wide range of plants because of their high polymorphism, abundance, and codominant inheritance. Different methods have been used for the analysis of the SSR amplified fragments being submarine agarose electropho...

BACKGROUND We previously reported that the one-step nucleic acid amplification assay is effective for lymph node metastasis detection in breast cancer patients. This paper describes the identification of CK19 mRNA as an optimal marker and its cut-off value for use in the detection of one-step nucleic acid amplification-based lymph node metastasis in colorectal cancer patients. METHODS Candida...

In this paper, digital quantitative detection of nucleic acids was achieved at the single-molecule level by chemical initiation of over one thousand sequence-specific, nanoliter isothermal amplification reactions in parallel. Digital polymerase chain reaction (digital PCR), a method used for quantification of nucleic acids, counts the presence or absence of amplification of individual molecules...

Nucleic acid amplification reactions offer a great deal of potential for the accurate and sensitive detectionlquantitation of microbial nucleic acids in human body fluids and have, in some cases, an edge over techniques using immunoassays. Successful detections include : nucleic acids from viruses such as HIV, rotaviruses, coxsackieviruses, papilloma viruses, pestiviruses as well as microorgani...

The sensitivity of PCR for the amplification of target nucleic acid sequences in clinical diagnostics may often be reduced due to the presence of inhibitory factors. Hemolytic serum contains a number of PCR inhibitors, one of which is hemin. In this study we have found that conventional methods of DNA extraction were not sufficient for the removal of PCR-inhibitory compounds in hemolytic serum....

Nucleic acid sequence-based amplification with electrochemiluminescent detection (NASBA/ECL) is an isothermal technique allowing rapid amplification and detection of specific regions of nucleic acid from a diverse range of sources. It is especially suitable for amplifying RNA. A NASBA/ECL technique has been developed allowing the detection of RNA from avian influenza virus subtype H7 derived fr...

The polymerase chain reaction (PCR) and Q/3 replicase are two methods in which nucleic acid polymerases are used for amplification. Although these approaches share many similar problems concerning target contamination and probe specificity, they differ dramatically in their mechanisms of action and modes of application.The PCR method amplifies target sequences between two priming oligonucleotid...

Novel methods were developed to isothermally detect target nucleic acids and initiate signal amplification cascades. The methods utilize target-specific MNAzymes to activate universal primer molecules. These primers can subsequently promote the autonomous synthesis of DNAzymes capable of cleaving nucleic acid substrates and generating signal, which further creates the potential for circular fee...