The immunomagnetic beads method for isolation of fetal nucleated red blood cells (FNRBCs) from peripheral blood of 78 pregnant women for prenatal diagnosis was developed. The study subjects were classified into 8-10 and 11-14 weeks of gestation (n = 39 each). Peripheral blood cells were divided into two for the FNRBCs isolation using two protocols, one with anti-CD45 depletion followed by anti-...

BACKGROUND Information on the prognostic utility of the admission complete blood count (CBC) and differential count is lacking. OBJECTIVE To identify independent predictors of mortality from the varied number and morphology of cells in the complete blood count defined as a hemogram, automated five cell differential count and manual differential count. DESIGN Retrospective cohort study and c...

In the capillary venules, blood cells auto-separate with red blood cells aggregating near the centre of vessel and the nucleated cells marginating toward the wall of vessel. In this experiment, we used cell margination to help enrich the Jurkat cells via a groove-based channel which provides a vertical expansion-contraction structure, wherein the red blood cells invade the grooves and push the ...

Fetal cells can be isolated using several enrichment methods, however, since no technique to specifically isolate and use them for genetic diagnosis has reached clinical practice, prenatal diagnosis currently requires invasive procedures. A micromachined device to fractionate whole blood using physical means to enrich for and/or isolate rare cell types from peripheral circulation was designed. ...

Background: People occasionally undergo medical diagnostic X-ray examinations and expose their red blood cells to radiation. Radiation that is generated from medical diagnostic X-ray machines is widely used in medical diagnoses. One of the important parameters is kilo-voltage (kV) that is applied across the X-ray tube in medical diagnostic X-ray machines. Kilo-voltage influences the radiation d...