In vitro propagation of AZ x M9 new apple rootstock resulted of breeding program of vegetative apple rootstock in Iran was investigated. Nodal explants were inoculated in Murashige and SKoog (MS) medium supplemented with various concentrations of 6-benzylaminon purine (BAP) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (NAA) (0.1 mg/l). We examined the effect of differe...

The present study aimed to develop a micropropagation protocol for Cadaman (P. persica x P. davidiana) and Garnem dulcis) peach rootstocks. Stem nodal segments with one axillary bud used as explants. MS medium supplemented different concentrations of BA or without IBA was studied determine the best  multiplication stage,  results revealed that 2.0 mg/l BA+0.1 optimal shoot proliferation rootsto...

In tissue culture, high production cost of the products restricts their reach. Though tissue culture is a major strength in floriculture it is marred by pricing issues. Hence, we developed a complete regeneration low cost micropropagation protocol for an economically important floriculture crop, carnation (Dianthus caryophyllus L.). Successful regeneration of carnation from nodal explants on co...

In this study Artemisia amygdalina a critically endangered medicinal plant endemic to Kashmir Himalayas was grown under in vitro conditions. ISSR markers were used to study the somaclonal variation at the genetic level. Two ISSR-primers (CAC)3 GC and (ACTG)5 were used, two additional DNA bands (0.15kb and 0.2kb) were observed respectively in tissue cultured Artemisia amygdalina. Direct regenera...

In this review, we report on the different plant growth regulators (PGRs) affecting callus induction, callus-mediated regeneration, organogenesis, somatic embryogenesis from explants and micropropagation through shoot tip nodal cultures in Turkey Berry (Solanum torvumSW). The fruits of guard against liver kidney damage, stop certain cancers, regulate blood sugar levels. They benefit digestion, ...

We developed procedures for the micropropagation of Juniperus oxycedrus L. using shoot apices or nodal segments from mature plants. Of the media and explants examined, best culture establishment was obtained with shoot apices cultured on modified Schenk and Hildebrandt medium (SH medium) without growth regulators; however, shoot multiplication was only achieved when shoot apices isolated from s...

Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P. chilensis were taken from 4 months-old plants grown in the greenhouse or from ...

In rubber, the current propagation method of grafting on to unselected seedlings, maintains intraclonal heterogeneity for vigour and productivity and hence a great improvement may be expected by using micropropagation in vitro. Micropropagation with nodal and shoot tip explants derived from seedlings are possible as with mature clonal explants. The major problem in using clonal material from ma...

An efficient and reproducible protocol for plantlet regeneration from nodal segments of Olive cv 'Frontio' has been developed. Media and explants browning due to exudation of phenolics from the explants were controlled by fortification of the medium with 100 mg/L ascorbic acid. Best establishment of olive explants was observed on half-strength MS salts fortified with 2.0 mg/L 6-benzylaminopurin...

a reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant vitex negundo through in vitro culture. of different concentrations of n6-benzyladenine (ba) evaluated as supplements to murahige and skoog (ms) medium, ba at 1mg/l was effective in enhancing bud break and shoot regeneration. response of explants was al...