Objective The purpose of this study was to investigate the effects of estradiol on the expression of hypoxia-inducible factor (HIF)-1α and the differentiation of trophoblasts in human first trimester villous explant cultures. Methods Villous explant cultures were established from first trimester human placentas (6-8 weeks of gestation, n=3). Normal villous tissues were explanted on Matrigel a...

This paper presents a method to allocate the transmission network costs to users based on nodal pricing approach by regulating the nodal prices from the marginal point to the new point. Transmission nodal pricing based on marginal prices is not able to produce enough revenue to recover the total transmission network costs. However, according to the previous studies in this context, this met...

PURPOSE A previous report has described an ocular surface reconstruction method involving the use of cultivated corneal epithelium derived from limbal explants. In the current study, a new culture system was developed involving the in vitro propagation on amniotic membrane (AM) of epithelial cells from enzymatically dissociated limbal epithelium. The purpose of this new method is to produce a c...

Introduction: Clinical examination and even anatomical imaging may fail to identify primary site of malignancy in patients presenting with cervical nodal metastasis. 18F-Fluorodeoxyglucose Positron Emission Computed Tomography (18F-FDG PET-CT) is known to overcome the limitations of anatomic imaging. Methods: Sixty-three (63) patient...

conclusions echocardiography and 2d speckle tracking strain imaging shows improvements in left ventricular deformation indices in atrioventricular reentrant tachycardia (avrt) and atrioventricular nodal reentrant tachycardia (avnrt) patients for the mean systolic strain and strain rate at 24 hours and the three-month follow-up. in spite of the normal lvef, even during sinus rhythm, deformation ...

PURPOSE The clinical success of treating corneas with total limbal stem cell deficiency using limbal biopsy explants cultured on intact amniotic membrane (iAM) relies on ex vivo expansion of limbal epithelial progenitor cells. However, the ultimate fate of limbal epithelial progenitor cells in the explant remains unclear. METHODS Human limbal explants were cultured on iAM for 2 weeks and then...

Many of the therapeutic interventions for intervertebral disc degeneration attempt to repopulate the nucleus pulposus (NP) tissue; however, NP cells are heterogeneous and not well characterized. To address this, we have investigated the morphology, extracellular gene and protein expression, and apoptosis changes in NP explants cultured in vitro with or without chondrogenic reagents for differen...

Background: Lilium (Lilium longiflorum Thunb.) belongs to the family Liliaceae and is a native of Northern Hemisphere (up South Canada Siberia). Conventionally, lilium can easily be propagated by sexual asexual methods propagation but these prevalent are not capable meeting increasing demand in domestic global market. Generally, through bulbs which has bottlenecks like high cost planting materi...

the present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (pgrs) combinations under in vitro conditions. the cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in ms medium containing ba (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and tdz (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...