The role of phytochrome in the induction of nitrate reductase of etiolated field peas (Pisum arvense L.) was examined. Terminal bud nitrate concentration increased in darkness, and the increase correlated with induction of nitrate reductase following brief exposure of intact plants to red, blue, far red, and white lights. Brief light exposure of intact plants stimulated nitrate uptake and induc...

The presence of nitrate in the growth medium of Escherichia coli causes the production of a multi-enzyme system capable of mediating electron transport between the existing respiratory chain and nitrate, resulting in its reduction to nitrite [for a review see Payne (1973)l. The point of interaction with the NADH-oxygen respiratory chain is probably at the level of ubiquinone (Enoch &Lester, 197...

Nitrate reductase from mutant 305 o f Chlamydomonas reinhardii has been purified about 90-fold and biochemically characterized. The enzyme can use reduced flavins and viologens as electron donors to reduce nitrate but, unlike the nitrate reductase com plex from its parental wild strain, lacks NAD(P)H-nitrate reductase and NAD(P)H-cytochrom e c reductase activities, does not bind to Blue-Agarose...

Demolybdo nitrate reductase (also called cyt c reductase) of Chlorella vulgaris has been converted to active nitrate reductase by insertion of Mo from Na2MoO4 in vitro. A procedure is described which consistently gives about 0.3 unit of nitrate reductase from about 6 units of cyt c reductase, a yield of 30% of the maximum expected, if we calculate on a basis of a ratio of 6 to 1 for the cyt c r...

Nitrate reductase-deficient barley (Hordeum vulgare L.) mutants were assayed for the presence of a functional molybdenum cofactor determined from the activity of the molybdoenzyme, xanthine dehydrogenase, and for nitrate reductase-associated activities. Rocket immunoelectrophoresis was used to detect nitrate reductase cross-reacting material in the mutants. The cross-reacting material levels of...

I would like to add to this well written article that we have also reported acquired methemoglobinemia cases with administration of cytanest in puerperal women with g6PD deficiency and infants in whom erythrocyte cytochrome 65 reductase was assoyed [1-4], which is the main enzyme for methemoglobin reductase in erythrocytes as mentioned by the authors. In addition, hereditary methemoglobinemias ...

NADH:nitrate reductase was extracted from corn leaves (Zea mays L. W64A x W182E) and purified on blue Sepharose. After the nitrate reductase was further purified by polyacrylamide gel electrophoresis, it was used to immunize mice and a rabbit. Western blots of crude leaf extracts were used to demonstrate monospecificity of the mouse ascitic fluids and the rabbit antiserum. The electrophoretic p...

The effect of nitrogen form (NH(4)-N, NH(4)-N + NO(3) (-), NO(3) (-)) on nitrate reductase activity in roots and shoots of maize (Zea mays L. cv INRA 508) seedlings was studied. Nitrate reductase activity in leaves was consistent with the well known fact that NO(3) (-) increases, and NH(4) (+) and amide-N decrease, nitrate reductase activity. Nitrate reductase activity in the roots, however, co...

Molybdenum is absolutely required for the nitrate-reducing activity of the nicotinamide adenine dinucleotide nitrate reductase complex isolated from Chlorella fusca. The whole enzyme nicotinamide adenine dinucleotide nitrate reductase is formed by cells grown in the absence of added molybdate, but only its first activity (nicotinamide adenine dinucleotide diaphorase) is functional. The second a...

Synthesis and turnover of nitrate reductase molecules and soluble proteins of tobacco XD cells were studied by labeling pre-existing proteins with 14C-arginine to make them radioactive and with 99 atom % ‘5N to increase their buoyant density. The cells were then transferred to medium in which new proteins were synthesized from 14N and were radioactively labeled with 3H-arginine. The breakdown o...