Introduction: A simple and reliable high performance liquid chromatographic method has been developed for the quantitative determination of pregabalin in bulk dosage form. Pregabalin, a γ amino butyric acid analogue, negligible sensitivity to UV or fluorescence detection. Hence, it derivatized by ninhydrin form chromophoric complex that could be quantified Materials Methods: The concentration w...

The introduction of ninhydrin treatment as a chemical technique for the visualisation of latent fingermarks on porous surfaces revolutionised approaches to forensic fingermark examination. Since then, a range of amino acid sensitive reagents has been developed and such compounds are in widespread use by law enforcement agencies worldwide. This paper reviews the development and use of these reag...

In the crystal of the title compound, C(12)H(8)N(2)O(4)S, mol-ecules are linked into chains by a series of inter-molecular O-H⋯O, N-H⋯O and N-H⋯N hydrogen bonds. The ninhydrin and amino-thia-zolidine units make a dihedral angle of 66.41 (3)°. The crystal structure indicates the presence of equimolar R and S enanti-omers in the crystal lattice, due to the presence of a chiral centre in the title...

Protein-free filtrates of blood treated with trichioracetic acid were divided into two or three parts. The ammonia concentration of one of these was determined immediately by the ninhydrin method described by Nathan and Rodkey (1). The others were kept frozen for periods of 1-8 days and then thawed and analyzed. The blood ammonia concentrations calculated from these filtrate values were compare...

1. The conditions required for sensitive and specific colorimetric determination of proline with acidified ninhydrin were investigated. 2. A method applicable to protein samples was developed. 3. The only compound found to interfere appreciably was a hydroxyproline.

The detection of small quantities of nitrogen-containing compounds on paper chromatograms is a problem of long standing interest. The ninhydrin technique, first introduced by Consden, Gordon, and Martin (l), has partially solved this problem and has proven to be extremely valuable. However, the narrow range of compounds detected, the decreased sensitivity with increased molecular weight of pept...