Two major species of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) differing in size, pyridine nucleotide specificity, and susceptibility to inhibition by adenosine 5'-triphosphate (ATP) were detected in extracts of Pseudomonas multivorans (which has recently been shown to be synonymous with the species Pseudomonas cepacia) ATCC 17616. The large species (molecular weight ca. 230,000) was acti...

An enzyme has been partially purified from brewer's yeast which catalyzes the reversible reaction between riboflavin phosphate and adenosine triphosphate (ATP) on the one hand and flavin-adenine dinucleotide (FAD) and inorganic pyrophosphate on the other in the presence of magnesium ions. ATP could not be replaced by adenosine diphosphate or adenosine-5-phosphate, and in the reverse reaction, i...

In this study, to reveal the antioxidant potential of goat milk cells, activities glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx) and levels reduced nicotinamide adenine dinucleotide phosphate (NADPH), total (tGSH),malondialdehyde (MDA), vitamin C (Vit C) protein (TP) in cells were determined correlations between these parameters evaluated. Milk samples collected from 19 ...

Various properties of the bacteriophage structural dihydrofolate reductase (DFR) have been examined to determine its function during phage infection. It has been found that a binding site for reduced nicotinamide adenine dinucleotide phosphate (NADPH), most likely on the DFR present in the phage tail plate, is required for phage viability. Attachment of adenosine diphosphoribose, an analogue of...

As part of an investigation of the growth of tomato plants supplied with either nitrate or ammonium nitrogen (6), leaves were assayed for NAD4 and NADP. Anderson and Vennesland (1) assayed NAD and NADP in various green tissues and reported recoveries between 50 % and 85 % of NAD and NADP added to spinach leaf extracts. Very low levels of NAD and NADP were recorded for tomato leaf extracts. Thes...

Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...

Glucose g-phosphate dehydrogenase was first described in yeast by Warburg and Christian (l), who showed that nicotinamide adenine dinucleotide phosphate was the specific coenzyme. The enzyme was later prepared from liver and again shown to be specific for the same coenzyme (2). Subsequent preparations (see Cheldelin (3)) from various microbial sources have included two which react with both nic...

Extensive research has been conducted to elucidate and substantiate the crucial role of Renin-Angiotensin System (RAS) in pathogenesis hypertension, cardiovascular disorders, renal diseases. Furthermore, oxidative stress maintaining vascular balance well established. It observed that many cellular effects induced by Angiotensin II (Ang II) are facilitated reactive oxygen species (ROS) produced ...

Glucose g-phosphate dehydrogenase was first described in yeast by Warburg and Christian (l), who showed that nicotinamide adenine dinucleotide phosphate was the specific coenzyme. The enzyme was later prepared from liver and again shown to be specific for the same coenzyme (2). Subsequent preparations (see Cheldelin (3)) from various microbial sources have included two which react with both nic...